Description
The ViPrimePLUS Human Parvovirus B19 qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of Human Parvovirus B19 genome in clinical samples (e.g. blood).
Human Parvovirus B19 (HPVB19) is a single stranded DNA virus which causes erythema infectiosum or commonly known as the fifth disease and hydrops fetalis. Rash on the cheeks is the defining symptom of fifth disease infection. It is very common in children and has a low mortality rate. Although healthy individuals usually recover from the infection without any major side-effects, immunocompromised patients may suffer from chronic anemia and other hematological abnormalities. In certain cases, neurological impairments may occur. Infected pregnant ladies suffer probably the most serious complication, especially during the first 20 weeks of the gestational period where non-immune hydrops fetalis and fetal death occur in 2-6% of the cases.
ViPrimePLUS Human Parvovirus B19 qPCR Kit is intended for research use only.
Kit Component
- HPVB19 Primers and Probe Mix (HPVB19 PIV-4b PPM)
- Positive Control
- Internal Extraction Control Primers and Probe Mix (IEC PPM)
- Internal Extraction Control (IEC DNA)
- Nuclease Free Water
- Template Preparation Buffer
Product Specification
| Technology | Taqman probe-based real-time PCR assay |
| Type of Nucleic Acid Kit | RNA |
| Kit Storage | Shipped in room temperature, store at -20℃ on arrival |
| Sample Material | Clinical samples (e.g. blood) |
| Gene Target | NS1 protein encoding gene |
| Detection Limit | 10 to <100 copies per reaction |
| Sensitivity & Specificity | Ct value 28±3 with probability of 95% |
| Controls Included | • Internal Extraction Control (IEC) as inhibition control • Positive control |
| Channels | • FAM channels detect pathogen amplicons • VIC/HEX channel detects IEC amplicons |
Ordering Information
| Catalog No | Description | Pack Size |
| QM5048 | ViPrimePLUS Human Parvovirus B19 qPCR Kit | 150 reactions |
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Manual
