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ViPrimePLUS Pseudomonas SPP qPCR Kit

Introduction

ViPrimePLUS Pseudomonas SPP qPCR Kit has designed to have very high homology with all reference data within the NCBI database. The kit has the broadest detection profile and detects all clinically relevant strains. The kit detects the Pseudomonas species; Pseudomonas aeruginosa,Pseudomonas fluorescens, Pseudomonas mendocina, and Pseudomonas putida. However, due to extreme homology,the primers may also detect Stenotrophomonas rhizophila and the following species previously classified as Pseudomonas: Stutzerimonas azotifigens, Stutzerimonas balearica, Stutzerimonas chloritidismutans, Stutzerimonas degradans, Stutzerimonas denitrificans, Stutzerimonas frequens, Stutzerimonas kunmingensis, Stutzerimonas stutzeri, Stutzerimonas xanthomarina and Stutzerimonas zhaodongensis.

Sensitivity & specificity

The detection limit is tested to have at least 100 copies per reaction. The primers and probe are 100% specific.

Kit Components

 

Kit ComponentsTube ColourVolume (ml)
Pseudomonas Primers and Probe Mix (gene PPM – FAM probe)Amber0.1 ml
Positive Control TemplatePinkLyophilized
Endogenous Control Primers and Probe Mix (EC PPM – HEX probe)Blue0.1 ml
2X PCR Master MixPurpleLyophilized
ROX Reference DyeBlack0.01 ml
Nuclease-free WaterWhite1.5 ml
Template Preparation BufferOrange1.5 ml
Master Mix Preparation BufferTransparent1.5 ml

Ordering Information

Catalog NoDescriptionPack Size
VECP019ViPrimePLUS Pseudomonas SPP qPCR Kit100 reactions