ViPrimePLUS Escherichia Coli qPCR Kit
Introduction
ViPrimePLUS Escherichia Coli qPCR Kit has designed to have very high homology with all reference data within the NCBI database. The kit has the broadest detection profile and detects all clinically relevant strains. The primers will detect all Escherichia coli strains but also may detect the species; Buttiauxella agrestis, Citrobacter youngae, Enterobacter asburiae, Enterobacter roggenkampii, Enterobacter soli, Escherichia fergusonii, Escherichia marmotae, Escherichia ruysiae, Kluyvera georgiana, Obesumbacterium proteus, Phytobacter diazotrophicus, Salmonella bongori, Salmonella enterica, Shigella dysenteriae, Shigella flexneri, Shigella sonnei, Shigella boydii and Trabulsiella odontotermitis due to extreme homology.
Sensitivity & specificity
The detection limit is tested to have at least 100 copies per reaction. The primers and probe are 100% specific.
Kit Components
| Kit Components | Tube Colour | Volume (ml) |
| Escherichia Coli Primers and Probe Mix (gene PPM – FAM probe) | Amber | 0.1 ml |
| Positive Control Template | Pink | Lyophilized |
| Endogenous Control Primers and Probe Mix (EC PPM – HEX probe) | Blue | 0.1 ml |
| 2X PCR Master Mix | Purple | Lyophilized |
| ROX Reference Dye | Black | 0.01 ml |
| Nuclease-free Water | White | 1.5 ml |
| Template Preparation Buffer | Orange | 1.5 ml |
| Master Mix Preparation Buffer | Transparent | 1.5 ml |
Ordering Information
| Catalog No | Description | Pack Size |
| VECE002 | ViPrimePLUS Escherichia Coli qPCR Kit | 100 reactions |
