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ViPrimePLUS Ehrlichia SPP qPCR Kit

Introduction

ViPrimePLUS Ehrlichia SPP qPCR Kit has designed to have very high homology with all reference data within the NCBI database. The kit has the broadest detection profile and detects all clinically relevant strains. The kit detects Ehrlichia SPP: Ehrlichia canis, Ehrlichia chaffeensis, Ehrlichia dumleri, Ehrlichia ewingii, Ehrlichia hainanensis, Ehrlichia japonica, Ehrlichia khabarensis, Ehrlichia maerkangensis, Ehrlichia minasensis, Ehrlichia muris, Ehrlichia ovina, Ehrlichia pampeana, Ehrlichia regneryi, Ehrlichia ruminantium, Ehrlichia shimanensis, Ehrlichia zunyiensis. Due to extreme homology, the kit may also detect some strains of: Allocryptoplasma californiense, Neoehrlichia arcana, Neoehrlichia australis, Neoehrlichia lotoris, Neoehrlichia mikurensis, Neorickettsia findlayensis, Neorickettsia risticii, Neorickettsia sennetsu.

Sensitivity & specificity

The detection limit is tested to have at least 100 copies per reaction. The primers and probe are 100% specific.

Kit Components

 

Kit ComponentsTube ColourVolume (ml)
Ehrlichia SPP Primers and Probe Mix (gene PPM – FAM probe)Amber0.1 ml
Positive Control TemplatePinkLyophilized
Internal Extraction Control Primers and Probe Mix (IEC PPM – HEX probe)Blue0.1 ml
Internal Extraction Control Template (IEC)Red Lyophilized
2X PCR Master MixPurpleLyophilized
ROX Reference DyeBlack0.01 ml
Nuclease-free WaterWhite1.5 ml
Template Preparation BufferOrange1.5 ml
Master Mix Preparation BufferTransparent1.5 ml

Ordering Information

Catalog NoDescriptionPack Size
VICE009ViPrimePLUS Ehrlichia SPP qPCR Kit 100 reactions