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T4 DNA Ligase

Description

T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA. This enzyme will join blunt-end and cohesive end termini as well as repair single stranded nicks in duplex DNA, RNA, or DNA-RNA hybrids.

Features

  • Ultrapure recombinant protein
  • Seals single-stranded nicks in duplex DNA, RNA or DNA-RNA hybrids.
  • ATP is an essential cofactor for the reaction.

Ordering Information

Catalog NoDescriptionPack Size
ME4303T4 DNA Ligase4000u
ME4304T4 DNA Ligase20000u

Publications

Mekloy Paktraporn et al. (2022) Investigation of genes coding for anti-HIV proteins MAP30 and MRK29 from Thai bitter melon (Momordica charantia L.). Pharm Sci Asia 2022; 49(1), 59-68

Mahboudi et al. (2018) Prospect and Competence of Quantitative Methods via Real-time PCR in a Comparative Manner: An Experimental Review of Current Methods, The Open Bioinformatics Journal, 11:1-11.

Mohandesi, N., Haghbeen, K., Ranaei, O., Arab, S.S., Hassani, S. (2017) Catalytic Efficiency and thermostability improvement of SUC2 invertase through rational site-directed mutagenesis. Enzyme and Microbial Technology. 96. pp14-22

Dehnaiv, E., Fathi-Roudsari,M., Mirzaie, S., Arab., S.S., Siadat, S.O.R., Khajeh, K. (2017) Engineering disulfide bonds in Selenomonas ruminantium B-xylosidase by experimental and computational methods. International Journal of Biological Macromolecules. 95. Pp..248-255

Busayapongchai, P., Siri, S.(2017). Sensitive detection of estradiol based on ligand binding domain of estrogen receptor and gold nanoparticles. Analytical biochemistry. 518, pp.60-68.

Meidaninikkjeh, S., Vaziri,F., Siadat, S.D. (2015) Cloning of conserved regions of nontypeable Haemophilus influenzae hmw1 core binding domain. International Journal of Molecular and Clinical Microbiology. 5(1) pp.510-515

Kohnehrouz, B.B., & Nayeri, S. (2016) Design, Cloning and In silico Analysis of Efficient siRNA-inducing Casette for Silencing Wheat γ-gliadins. Jordan Journal of Biological Sciences, 9(1), p.35-40.

ME4303_T4 DNA Ligase, 4000 units
ME4304_T4 DNA Ligase, 20000 units
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