Ribonuclease A (RNase A)
Description
RNase A is an endoribonuclease that is from bovine pancreas for molecular biology applications. The major application for RNase A is the removal of RNA from preparation of plasmid DNA as well as extraction of plasmid DNA. It is also used in removal of unspecifically bound RNA; RNase protection assays; analysis of RNA sequences as well as hydrolysis of RNA contained in protein samples.
Source
Bovine pancreas
All products from animal sources are produced from starting material of USDA-approved origin, collected in United States Department of Agriculture (USDA) or equivalent approved facilities, inspected to be free of disease and suitable for exportation. Certificates of Origin are available upon request.
Unit Definition
One unit according to Kunitz produces an increase in absorbance of 0.001/minute under assay conditions in 1ml at 260nm.
Specification
| Assay | Result |
| Activity (Kunitz) | ≥60 units/mg protein |
| Molecular mass | 13.7 kDa (amino acid sequence) |
| Extinction coefficient | E1% = 7.1% (280nm) |
| Isoelectric point | pI: 9.6 |
| Optimum temperature | 60°C (activity range of 15 - 70°C) |
| Optimum pH | 7.5 (activity range of 6 - 10) |
| Inhibitors | Ribonuclease inhibitor |
| Loss on drying | ≤5.0% |
| DNase | None detected |
| Solubility (1% water) | Pass |
| Chloride | ≤0.014% |
| Sulfate | ≤0.15% |
| Heavy metals | ≤0.002% |
| Arsenic | ≤8ppm |
| Aluminium, Ca & related elements | Pass |
| Residual solvents | Pass |
Ordering Information
| Catalog No | Description | Pack Size |
| PC0713-250mg | Ribonuclease A (RNase A) | 250mg |
| PC0712-500mg | Ribonuclease A (RNase A) | 500mg |
| PC0712-1g | Ribonuclease A (RNase A) | 1g |
| PC0712-1ml | Ribonuclease A (RNase A) | 1ml |
