DL-Dithiothreitol (DTT)
Grade
Ultra Pure Grade
Description
An excellent reagent for maintaining SH groups in reduced state; quantitatively reduces disulfides. DTT is effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N’-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. DTT is less pungent and is less toxic than 2-mercaptoethanol.
Storage
Store at -20°C
Specification
| Appearance | Mol. Formula | Mol. Weight | CAS RN |
| White powder | C4H10O2S2 | 154.25 | 3483-12-3 |
| Assay | Specifications |
| Titration ex –SH (iodometric) | ≥99.0% |
| T.L.C. | One spot |
| UV Assay | |
| 1% Solution A280 | <0.067 |
| A305 | <0.050 |
| 0.02M Solution A283 | <0.040 |
| 1M Neutral Solution A400 | <0.062 |
| A405 | <0.062 |
| OD 700-500nm | ≤0.035 |
| Loss on Drying | <0.5% |
| Melting Point | 42±2°C |
| Oxidized DTT | <0.5% |
| Solubility (10% Water) | Clear and colorless |
| I.R. | Consistent with assigned structure |
| DNase | None |
| RNase | None |
| Protease | None |
Ordering Information
| Catalog No | Description | Pack Size |
| PC0705 - 5g | DL-Dithiothreitol (DTT) | 5g |
| PC0705 - 25g | DL-Dithiothreitol (DTT) | 25g |
| PC0705 - 100g | DL-Dithiothreitol (DTT) | 100g |
