Description
ViPrimePLUS Lyophilized One Step RT-qPCR Master Mix has been improved and upgraded for a fast and easy real-time PCR reaction set up. The master mix has been freeze-dried into beads form in order to have ambient shipping and produce a room temperature stable preparation. The improved formulation of master mix contains antibody mediated Hot Start Taq DNA Polymerase, unique thermostable reverse transcriptase and the highest quality buffer components at optimal concentrations. The master mix provides the mechanism to achieve excellent results in reaction efficiency, correlation coefficient and slope.
ViPrimePLUS Lyophilized qPCR Master Mix can be used to amplify any RNA template including mRNA, total RNA and viral sequences. The improved sensitivity and consistency of ViPrimePLUS Lyophilized One Step RT-qPCR Master Mix in standard cycling conditions allow for industry leading performance in fast cycling conditions.
Applications
All kinds of RNA sample material suited for one step RTqPCR amplification can be used.
Features
- Lyophilized form – ambient stable preparation
- Fast and easy real-time PCR reaction set up
- Highest sensitivity and specificity
- Compatible on most of the real-time PCR platforms
Kit Component
48 reactions / pack; 6 strips of 8 rxns/strip
96 reactions / pack; 12 strips of 8 rxns/strip
Storage & Shipment
Store at 2-8°C on arrival; store at dry and cool area with silica pack and stable inside sealed package up to the expiry date. Ship at ambient temperature within 10 days. Once reconstitution, the master mix should be used immediately; or stored at -20°C within a month.
Intended Use
For research use only. Not registered for diagnosis use.
Master Mixes Selection Guidelines for System Compatibility
To calibrate a real-time PCR reaction, the various formulations of Master Mixes are available for most of the platforms.
| Components | Reaction (1X) |
| Step RT-qPCR Master MixViPrimePLUS Lyophilized One | 1 tube |
| Primers (6pmols Forward & Reverse) | 1 μl per type |
| Probe (3pmols) | 1 μl |
| Template (10-100ng) | 1-5 μl |
| Nuclease-free water | Top up to 25 μl |
Ordering Information
| Catalog No | Description | Pack Size |
| QLMM22-48 | ViPrimePLUS Lyophilized One Step qRT-PCR Master Mix | 48 reactions |
| QLMM22-96 | ViPrimePLUS Lyophilized One Step qRT-PCR Master Mix | 96 reactions |
Download
Manual
ViPrimePLUS Lyophilized One Step qRT-PCR Master Mix (48 reactions)
ViPrimePLUS Lyophilized One Step qRT-PCR Master Mix (96 reactions)
Publication
This Product Has Been Used In:
Petra Drzewnioková et al. (2025) Validation and multi-site deployment of a lyophilized qRT-PCR reagent for the molecular diagnosis of avian influenza and rabies in Sub-Saharan African regions Journal of Clinical Microbiology , 2025-07
