Description
ViPrimePLUS Lyophilised One Step qRT-PCR Master Mix is next generation first choice mix for a fast and easy one step real-time PCR reaction set up. The master mix has been freeze-dried to produce a room temperature stable preparation. The improved formulation of lyophilized master mix contains unique thermostable M-MULV (Moleney murine leukemia virus) enzyme, Taq DNA Polymerases as well as MgCl2 and buffer components at optimal concentrations. The M-MULV enzyme has an optimal operating temperature of 55°C, and has a higher affinity for primer template duplexes which allows very rapid processing during RT step. The One Step qRTPCR Master Mix is designed to achieve excellent results in reaction efficiency, correlation coefficient and slope.
ViPrimePLUS Lyophilized One Step qRT-PCR Master Mix can be used to amplify any RNA template including mRNA, total RNA and viral sequences. The improved formulation of qRT-PCR can detect the extremely low copy number targets very specifically with high efficiency that give CT values close to the theoretical time of detection. The ViPrimePLUS Lyophilized One Step qRT-PCR Master Mix is complete system for use in one step real-time PCR; the removal of a separate reverse transcription step reduces handling errors as well as the time taken to obtain results. The formation of primer dimers and non-specific products is prevented leading to optimum sensitivity and specificity.
ViPrimePLUS Lyophilized One Step qRT-PCR Master Mix supplied with re-suspension buffer and ROX dye to be used with most of real-time PCR instruments which include hardware platforms that need ROX as a passive reference dye. The improved sensitivity and consistency of ViPrimePLUS Lyophilized One Step qRT-PCR Master Mix in standard cycling conditions allow for industry leading performance in fast cycling conditions.
Features
- Lyophilized form – room temperature stable preparation
- Fast and easy Real-Time PCR reaction set up
- Rapid extension rate for early Ct values
- Highest sensitivity and specificity
- Increased limit of detection
- Compatible on most of the real-time PCR platforms
Kit Component
- 3 x Lyophilized One Step qRT-PCR Master Mix
- 1 x Lyophilized ROX
- 4 x Lyophilized ROX
Master Mixes Selection Guidelines for System Compatibility
To calibrate a real-time PCR reaction, the various formulations of Master Mixes are available for most of the platforms.
| Real-time PCR platforms | ROX re-suspension volume | ROX addition per tube of master mix |
| Applied Biosystems 7000, 7700, 7900, 7300 StepOne, StepOnePLUS and ViA7 platforms, Roche capillary Lightcyclers. | 100µl | 20µl |
| All Stratagene platforms | 200µl | 15µl |
| Applied Biosystems 7500 platform | 700µl | 10µl |
| All other machines | Not Required | Not Required |
Ordering Information
| Catalog No | Description | Pack Size |
| QLMM06 | ViPrimePLUS Lyophilized One Step qRT-PCR Master Mix | 150 reactions |
Download
Manual
ViPrimePLUS Lyophilized qPCR Master Mix
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MSDS
ViPrimePLUS Lyophilized qPCR
