Grade
Ultra Pure Grade
Description
CHAPS is a standard reagent suitable for use as a solubilizing agent in isoelectric focusing applications is tested to meet low metal content specifications.
CHAPS is a non-denaturing, zwitterionic sulfobetaine detergent commonly used protein solubilization in isoelectric focusing and two-dimensional electrophoresis as well as used in breaking protein-protein interactions. CHAPS has been shown to give excellent resolution of some subcellular preparations and plant proteins. Concentrations between 1-4% (w/v) are typically used in an IEF gel. A commonly used IEF sample solution consists of 8M urea, 4% CHAPS, 50-100mM dithiothreitol (DTT), and 40mM Tris. Furthermore, its small micellar molecular weight (6150) and high critical micellar concentration (6-10mM) allow it to be removed from samples by dialysis.
The preparation of CHAPS is 50mg/ml. CHAPS is soluble in water.
Storage
Store at RT
Specification
| Appearance | White to pale white powder |
| Mol. Formula | C32H58N2SO7 |
| Mol. Weight | 614.89 |
| CAS RN | 75621-03-3 |
| Assay | Specifications |
| Purity | ≥99.0% |
| Loss on drying | ≤4.0% |
| TLC | One spot |
| Solubility (10% aq.) | Clear, colorless solution |
Ordering Information
| Catalog No | Description | Pack Size |
| PC0925-5g | 3-[(3-cholamidopropyl)dimethyl-ammonio]-1- propanesulfonate (CHAPS) | 5g |
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MSDS
3-[(3-cholamidopropyl)dimethyl-ammonio]-1- propanesulfonate (CHAPS)
