Grade
Ultra Pure Grade
Description
DNase I is a recombinant form of DNase I which is RNase-free, originally isolated from bovine pancreas, is a recombinant enzyme expressed in Pichia pastoris. It is a DNA-specific endonuclease that hydrolyzes the phosphodiester linkages of double- and single-stranded DNA to a mixture of mono- and oligonucleotides.
DNase I manufactured using state-of-the-art processes yielding animal-free material. The enzyme is highly purified and rigorously tested for contaminating RNase and protease activity of RT-PCR. It is an important tool for all applications requiring DNA-free RNA templates and achieving reliable results with undegraded and stable RNA.
Source
Bovine pancreas
Unit Definition
One unit according to Kunitz produces an increase in absorbance of 0.001/minute under assay conditions in 1ml at 260nm.
Specification
| Assay | Resuls |
| Volume act. with calf thymys DNA (Kunitz) | 11875 u/ml |
| Volume act. with calf thymys DNA (Kunitz) | 993 KU/ml |
| Protease up to 50U w.casein resorufinm |
Not detectable. |
| RNases up to 10U with MS II RNA |
Not detectable. |
Storage
Store at -20°C
Ordering Information
| Catalog No | Description | Pack Size |
| PC0719 - 50kU | Deoxyribonuclease I (DNase I) - For RNA work | 50kU |
| PC0719 - 100kU | Deoxyribonuclease I (DNase I) - For RNA work | 100kU |
Download
Manual
Deoxyribonuclease I (DNase I) - For RNA work
Download
MSDS
