The first strand DNA was synthesized using random hexamers (Vivantis, Malaysia) in a total reaction volume of 25 μl using M-MLV reverse transcriptase (Vivantis, Malaysia). The cDNA products were immediately used for RT-PCR or real-time PCR by a research group from Iran involved in the research of DNA methylation and histone acetylation patterns in cultured bovine adipose tissue-derived stem cells (BADSCs)
View Paper | View Product |