The first strand cDNA was synthesized using random hexamers (Vivantis, Malaysia) in a total reaction volume of 25 µl using M-MLV reverse transcriptase (Vivantis, Malaysia). The cDNA products were immediately used for RT-PCR or real-time PCR by researchers from Iran involved in the research of DNA Methylation and Histone Acetylation Patterns in Cultured Bovine Adipose Tissue-Derived Stem Cells (BADSCs).
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