Leaves samples were collected from the diseased trees to isolate DNA and to confirm greening pathogen with two primer pairs, which amplified the specific DNA (~1160bp) and βoperon (~703bp) regions. DNA was extracted with the GF-1 Plant II DNA Extraction Kit from Vivantis by a research group from Pakistan who investigate the Incidence and molecular detection of greening disease in two citrus cultivars in Sargodha, Pakistan.
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