14 Feb 2017
Vivantis is honored to announce the release of Fast Digest Restriction Endonucleases – hosted with 24 enzymes under Restriction Endonuclease product line for rapid DNA digestion within 5 to 15 minutes.
Vivantis Fast Digest Restriction Endonucleases are the advanced version of Vivantis restriction endonucleases - designed for rapid DNA digestion within 5-15 minutes. The fast digest restriction endonucleases are suitable for complete digestion of different DNA types, including lambda DNA, plasmid DNA, genomic DNA as well as downstream PCR products.
Each Fast Digest Restriction Endonuclease is supplied with universal buffer, Buffer FD (Buffer Fast Digest), which does not limit multiple digestions of the Fast Digest Restriction Endonucleases. All Fast Digest Restriction Endonucleases are 100% active in Buffer FD, which allows any combination of Fast Digestion Restriction Enzymes to work simultaneously in one reaction tube.
Fast Digest Restriction Endonucleases are experimentally tested and proven to have the same performance as per conventional restriction endonucleases – with lesser reaction times which greatly improve restriction digestion efficiency.
APPLICATIONS:
- Digestion of different types of DNAs
- Digestion of PCR products
- RFLP genotyping
- Clone analysis
Hassle-Free Restriction Digestion within 5-15 Minutes
- NO MORE long incubation time and overnight digestions
- NO MORE various color tag buffer system
- NO MORE dilution
- NO MORE star activity
Summary Table of Incubation Time of Vivantis Fast Digest Restriction Endonucleases
| Cat. No. | Fast Digest Restriction Endonuclease | Incubation time (mins) in 20μl reaction volume | Incubation time (mins) in 50μl reaction volume | ||||
| 5 | 10 | 15 | 5 | 10 | 15 | ||
| FDRE1104 | Acc65 I | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ |
| FDRE1118 | Ahl I | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ |
| FDRE1120 | Alu I | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ |
| FDRE1124 | Apa I | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ |
| FDRE1126 | AsiG I | ✕ | ✓ | ✓ | ✕ | ✓ | ✓ |
| FDRV1138 | BamH I | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ |
| FDRV1144 | Bgl II | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ |
| FDRE1226 | BstMA I | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ |
| FDRE1260 | EcoR I | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ |
| FDRE1262 | EcoR V | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ |
| FDRE1266 | FauND I | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ |
| FDRV1276 | Hind III | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ |
| FDRE1278 | Hinf I | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ |
| FDRE1280 | Hpa I | ✕ | ✓ | ✓ | ✕ | ✓ | ✓ |
| FDRE1294 | Mlu I | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ |
| FDRE1308 | Pce I | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ |
| FDRE1312 | Psp124B I | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ |
| FDRE1320 | Pst I | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ |
| FDRV1326 | Sal I | ✕ | ✕ | ✓ | ✕ | ✓ | ✓ |
| FDRE1332 | Sfr274 I | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ |
| FDRE1336 | Sma I | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ |
| FDRV1340 | Sph I | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ |
| FDRV1364 | Xba I | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ |
Comparison between Vivantis Restriction Endonucleases and Vivantis Fast Digest Restriction Endonucleases
| Vivantis Restriction Endonuclease | Vivantis Fast Digest Restriction Endonuclease (FDRE) | |
| Incubation time | 1 hour – 16 hours | 5 – 15 minutes |
| Buffer system |
Up to 15 color-tag reaction buffers
(eg. Buffer V1, V2, UB1x, etc)
|
One universal buffer (Buffer FD)
|
| Double/multiple restriction digestion | Limited due to buffer incompatibility | No limit – all 24 Fast Digest Restriction Endonucleases are 100% active in one universal buffer |
| Dilution step | Dilution is optional depending on experiment | NO dilution required |
| Activity definition | 1 unit of restriction enzyme is required to digest 1ug of DNA in 1 hour at optimum temperature in 50µl of assay buffer. | 1µl of restriction enzyme is required to digest 1µg of DNA within 15 minutes at optimum temperature in 20µl or 50µl of assay buffer. |
