Concentration
5-10u/μl
5'…TCG↓CGA…3'
3'…AGC↑GCT…5'
Reaction Conditions
1X Buffer V4
10mM Tris-HCl (pH 8.5 at 30°C), 10mM MgCl2, 100mM KCl, and 100μg/ml BSA. Incubate at 37°C.
Storage Buffer
10mM Tris-HCl (pH 7.5), 250mM NaCl, 0.1mM EDTA, 7mM 2-mercaptoethanol, and 50% glycerol. Store at -20°C.
Thermal Inactivation
None.
Ligation / Recutting Assay
After 10-fold overdigestion with BtuM I, about 50% of the DNA fragments can be ligated and of these 90% can be recut.
Overdigestion Assay
An unaltered banding pattern was observed after 1μg of DNA was digested with 5u of BtuMI for 16 hours at 37°C.
Supplied with 10X Buffer V4, 10X Buffer UB and Viva Buffer A. (Diluent)
*Blocked by overlapping dam-methylation (GmATC): GATCGCGA, (TCGCGATC).
![](/images/stories/prod_image/restriction_endonucleases/btumi nrui.jpg)
Ordering Information
Catalog No | Description | Pack Size |
RV1246 | BtuMI {NruI} | 200u |
Download
Manual
BtuMI {NruI}
Download
MSDS
BtuMI {NruI}