Vivantis Technologies Sdn Bhd

Concentration
1-2u/μl

5'…R↓CCGGY…3'
3'…YGGCC↑R…5'

Reaction Conditions
1X Buffer V3
50mM Tris-HCl (pH7.5 at 30°C), 10mM MgCl₂, 100mM NaCl and 100µg/ml BSA. Incubate at 65°C.

Storage Buffer
10mM KH₂PO₄ (pH 7.5), 100mM NaCI, 0.1mM EDTA, 7mM 2-mercaptoethanol, 100μg/ml BSA and 50% glycerol. Store at -20°C.

*Enzyme is stable for up to 6 months if properly stored. It is recommended that the enzyme is kept in small aliquots to avoid repeated freeze-thaw cycles.

Thermal Inactivation
None.

Ligation / Recutting Assay
After 2-fold overdigestion with Bse118I, 95% of the DNA fragments can be ligated and recut.

Overdigestion Assay
An unaltered banding pattern was observed after 1μg of DNA was digested with 2u of Bse118I for 16 hours at 65°C.

Supplied with 10X Buffer V3, 10X Buffer UB and Viva Buffer A. (Diluent)

Ordering Information

Download
Manual
Bse118I {Cfr10I}

Download
MSDS
Bse118I {Cfr10I}