Description
Viva cDNA Synthesis Kit is specially designed to provide reliable synthesis of full-length cDNA. M-MuLV RNase H- synthesizes complementary DNA strand initiating from a specific primer, oligo d(T) or random hexamer. The absence of RNase H enhances the synthesis of long cDNA as the RNA strand does not degrade in DNA-RNA hybrid during first strand cDNA synthesis. This cDNA synthesis kit is readily compatible with various cDNA-dependent downstream applications.
Features
- Absence of RNase H activity allows high yield of full length cDNA synthesis with RNA templates up to 10kb
- Wide selection of primers, oligo d(T) or random hexamer
- Highly compatible with various downstream applications
- Allows synthesis of full length cDNA from various RNA templates
- High capacity and able to copy up to 2μg of purified mRNA
Applications
- Traditional PCR amplification or real-time PCR quantification
- Storage of selective and functional genetic information by removing of introns
- Cloning of the gene of interested enables horizontal gene transfer (HGT)
- cDNA probe library construction with large and full-length inserts
- cDNA microarray for gene discovery and clinical diagnosis via gene pattern screening
- mRNA expression studies and storage of proteins material at genomics level
Quality Control
All preparation is assayed for contaminating endonucleases, exonucleases and non-specific RNase activities.
| Kit Components | 50 reactions | 100 reactions |
| M-MuLV Reverse Transcriptase | 5,000u | 10,000u |
| 10X Buffer M-MuLV | 125μl | 0.25ml |
| 10mM dNTPs mix | 125μl | 0.25ml |
| Oligo d(T)18 (40μm) | 50 μl | 100μl |
| Random hexamer (50ng/μl) | 50 μl | 100μl |
| Nuclease-free water | 1ml | 1ml x 2 |
Storage & Stability
Stable at -20°C for 18 months if reagents are properly stored.
| Catalog No | Description | Pack Size |
| cDSK01-050 | Viva cDNA Synthesis Kit | 50 reactions |
| cDSK01-100 | Viva cDNA Synthesis Kit | 100 reactions |
Download
Manual
Viva cDNA Synthesis Kit-50 reaction
Viva cDNA Synthesis Kit-100 reaction
Publication
This Product Has Been Used In:
Toraj Z-N et al. (2021) Expression of cyclin D1, p21 and estrogen receptor alpha in aflatoxin G1-induced disturbance in testicular tissue albino mice, Research in Pharmaceutical Science, 16(2):182-192.
Zeymep CO et al. (2021) Whole blood miR-210, miR-122, miR-223 expression levels and their relationship with iron status parameters and hypercoagulability indices in children with iron deficiency anemia, Journal of Pediatric Hematology/Oncology 43(3).
Ahmad Asnawi MUS et al. (2020) Total RNA extraction from the aromatic Phalaenpsis bellina, endemic orchid in Sabah, Borneo, Walailak Journal of Science & Technology, 18(1):6553.
Azad, A.R. et al. (2020) The Prevalence of Hepatitis B and D Viruses and Evaluating YMDD Mutation in HBV-Suspected Patients in Qom Province, Iran, Jundishapur Journal of Microbiology, 13(2):e100038.
Barati et al. (2020) 1Hz 100mT Electromagnetic Field Induces Apoptosis in Breast Cancer Cells Through Up-Regulation of P38 and P21, Multidisciplinary Cancer Investigation, 4(1)23-29.
Gholamreza Bahrami et al. (2020). Molecular mechanism of the anti-diabetic activity of an identified oligosaccharide from Rosa canina, . Research in Pharmaceutical Sciences, 15(1): 36-47
Gharibi A. & Andouhjerdi R.B. (2019) Evaluation of the Expression of the CK-18 Tumor Marker in Blood Samples of Breast Cancer Patients using Real-Time PCR,. International Journal of Advanced Biological and Biomedical Research, 7(2):142-150.
Pazhouhi, M., Sariri, R., Rabzia, A., Khazaei, M. (2016). Thymoquinone synergistically potentiates temozolomide cytotoxicity through the inhibition of autophagy in U87MG cell line, Iranian Journal of Basic Medical Sciences, Vol. 19, 890-898 (2016).
Alavijeh, M.G., Moslemi, E., Izadi, A. (2014). Study the expression of estrogen receptor alpha in women with breast cancer, Bulletin of Environment, Pharmacology and Life Sciences, Vol. 3, No. 12 (2014).
