AtTaq DNA Polymerase (Hot Start)

Description
AtTaq DNA Polymerase is a complex of specific anti-Taq monoclonal antibody with top quality thermostable Taq DNA Polymerase for automatic "Hot Start" amplification, resulting in greatly enhanced amplification specificity, sensitivity and yield. AtTaq DNA Polymerase catalyzes the polymerization of nucleotides into duplex DNA in the 5' to 3' direction with the presence of Mg²+ and has the 5' to 3' exonuclease activity.

Features

Ultra pure recombinant protein which is reversibly complex with anti-Taq monoclonal antibody that blocks replication activity of the enzyme at moderate temperatures.
Carefully selected anti-Taq antibodies have high thermal stability, providing protection against non-specific primer extension from room temperature to 70°C.
Formation of complexes between Taq DNA Polymerase and an anti-Taq antibody forms a basis for automatic “Hot Start” amplification, which allows for the assembly of amplification reactions at room temperature.
High stability of the complexes allows enormous increase in amplification specificity, sensitivity and yield in comparison to the conventional amplification assembly method.
Increased specificity as a result of reduced amplification artefacts such as primer-dimer formation and mispriming in multiplex amplification.
10X ViBuffer S provided for amplification of more than 5kb amplicon.

Unit Definition
1u is defined as the amount of enzyme that is required to catalyze the incorporation of 10nmol of dNTP into acid-insoluble material in 30 minutes at 74°C. The reaction conditions are: 50mM Tris-HCl (pH 9.0 at 25°C), 50mM NaCl, 5mM MgCl₂, 200μM each of dATP, dCTP, dGTP, dTTP (a mix of unlabeled and [³H]dTTP), 10μg activated calf thymus DNA and 100μg /ml BSA in a final volume of 50μl.

Supplied With

10X ViBuffer A (without MgCl₂ )
500mM KCl, 100mM Tris-HCl (pH 9.1 at 20°C) and 0.1% Triton™ X- 100. The buffer is optimized for use with 0.1-0.2mM of each dNTP.
10X ViBuffer S
160mM (NH₄)₂SO₄ , 500mM TrisHCl (pH 9.2 at 22°C), 17.5mM MgCl₂ and 0.1% Triton™ X-100. The buffer is optimized for use with 0.35mM of each dNTP.
50mM MgCl₂

Quality Control
All preparations are assayed for contaminating endonuclease, exonuclease, and non-specific DNase activities. Functionally tested in DNA amplification.

Storage Buffer
20mM Tris-HCl (pH 8.0 at 22°C ), 100mM KCl, 0.5% Tween™ 20, 0.5% Nonidet- P40, 0.1mM EDTA, 1mM DTT, and 50% glycerol. Store at -20°C.