Vivantis Technologies Sdn Bhd

Description
Chromo MaxTaq DNA Polymerase is a modified and optimized thermostable enzyme blend containing Taq DNA Polymerase, Pfu DNA Polymerase and enhancing factors. It exhibits the 3' to 5' proofreading activity, resulting in considerably higher amplification fidelity than possible with unmodified Taq DNA Polymerase. The enzyme is supplemented with inert color tracer dyes. Recommended for use in amplification to obtain DNA products up to 20kb.

Features

• Color tracer dyes for ease of visualization of the addition of polymerase to the reaction.
• Use of 1u in every 25μl final reaction allows direct loading and serves as tracking dyes during gel electrophoresis. The blue and pink color dyes migrate approximately at 4kb and 0.3kb respectively on 1% TAE agarose gel.
• Ultra pure recombinant protein allows amplification up to 20kb.
• 10X ViBuffer S provided for amplification of more than 5kb amplicon.
• Excellent for multiplex amplification as it exhibits wider tolerance for Mg2+ and salt concentrations.
• Improves amplification results with critical templates, such as those containing GC-rich regions, palindromes or multiple repeats.
• Increased amplification product yields and purity.
• Generates a mixture of blunt end and 3' dA overhang amplification products, majority of the products are blunt ended.

Unit Definition
1u is defined as the amount of enzyme that is required to catalyze the incorporation of 10nmol of dNTP into acid-insoluble material in 30 minutes at 74°C. The reaction conditions are: 50mM Tris-HCl (pH 9.0 at 25°C), 50mM NaCl, 5mM MgCl₂, 200μM each of dATP, dCTP, dGTP, dTTP (a mix of unlabeled and [³H]dTTP), 10μg activated calf thymus DNA and 100μg/ ml BSA in a final volume of 50μl.

Supplied With

• 10X ViBuffer A (without MgCl₂)
  500mM KCl, 100mM Tris-HCl (pH 9.1 at 20°C) and 0.1% Triton™ X-100. The buffer is optimized for use with 0.1-0.2mM of each dNTP.
• 10X ViBuffer S
  160mM (NH₄)₂SO₄, 500mM TrisHCl (pH 9.2 at 22°C), 17.5mM MgCl₂ and 0.1% Triton™X-100. The buffer is optimized for use with 0.35mM of each dNTP.
• 50mM MgCl₂

Quality Control
All preparations are assayed for contaminating endonuclease, exonuclease, and non-specific DNase activities. Functionally tested in DNA amplification.

Storage Buffer
20mM Tris-HCl (pH 8.0 at 22°C), 100mM KCl, 0.5% Tween™ 20, 0.5% Nonidet- P40, 0.1mM EDTA, 1mM DTT, color dyes and 50% glycerol. Store at -20°C.

Ordering Information

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Manual

Chromo MaxTaq DNA Polymerase

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MSDS

Chromo MaxTaq DNA Polymerase

Publication
This Product Has Been Used In:

Fausto, A.G.C., García, J.R.G., Madero, L.E.W.L., Torres, M.T.M. (2016) ) Two novel mutations in the ABCG5 gene, c.144 -1G>A and c.1523 delC, in a Mexican family with sitosterolemia. Journal of Clinical Lipidology. 10(1). pp.204-208

Khalafalla, A.I., Al-Busada, K.A., & El-Sabagh, I.M. (2015) Analysis of Naturally Occuring Resistant Mutations to Hepatitis C Virus NS3 Protease Inhibitors Virology Journal. 12(102), p. 1-10.