Vivantis Technologies Sdn Bhd

Description
HS Bst DNA Polymerase is the recombinant DNA polymerase enzyme obtained from the large fragment of Bacillus stearothermophilus DNA Polymerase by directed genetic engineering. It contains the 5’→ 3’ DNA polymerase activity and strong strand displacement activity, but lacks 5’→ 3’ exonuclease activity. HS Bst DNA Polymerase combines a new generation of HOT START technology to inhibit polymerase activity at temperatures below 50℃ and release polymerase activity at temperatures above 50℃. The reaction system can be prepared at room temperature. Compared with wild-type, HS Bst DNA Polymerase with Hot Start is suitable for isothermal amplification, featuring high amplification ability with superior amplification speed, high specificity and thermal stability. This product does not have supplied isothermal reaction buffer; suitable to be used for isothermal DNA amplification with different tests designs.

Features

• Suitable for colorimetric isothermal amplification (LAMP)
• Large Fragment
• Hot Start technology
• Superior amplification speed
• Supplied with needed reaction Buffer

Unit Definition:
1 unit (U) is defined as the amount of the enzyme that is required to incorporate 10 nmol of dNTPs into an acid-insoluble material in 30 minutes at 65℃.

Storage Buffer:
10 mM Tris-HCl, 50 mM KCl, 0.1 mM EDTA, 50% (v/v) glycerol, 0.1% Triton®X-100.

10X Amplification Buffer*:
100 mM (NH4)₂SO₄, 500 mM KCl, 20 mM MgSO₄, 1% Tween®20.
*This buffer is pH-sensitive.
* Prepare small aliquot of buffer before use to avoid repeated freeze thaw cycles.
100 mM MgSO₄

Application:
Isothermal amplification (LAMP), HDA (Helicase-Dependent Amplification) and RCA (Rolling Circle Amplification) and related research.

Quality Control:
All preparations are assayed for contaminating endonuclease, exonuclease and non-specific DNase activities. Functionally tested in DNA amplification.

Ordering Information

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Manual

HS Bst DNA Polymerase