Description
2X ViRed Pfu Master Mix is an optimized ready-to-use 2X concentrated DNA amplification mixture premixed with red color tracking dye. The ViRed Pfu Master Mix contains Pfu DNA Polymerase, reaction buffer, dNTPs, inert red dye and stabilizers needed for routine DNA amplification to obtain a wide range of PCR and DNA products up to 8kb. An inert red dye and stabilizers allows direct loading of final products onto gels for electrophoresis. The red color dye migrates at approximately 400bp on 1% agarose in 1X TBE Buffer. Pfu DNA Polymerase is an extremely thermostable proofreading DNA polymerase. It exhibits the 3’ to 5’ proofreading activity, resulting in over 10-fold higher fidelity than possible with Taq DNA Polymerases.
Features
- Suitable for all routine DNA amplification applications
- Recommended for use in high-fidelity amplification, amplification of GC-rich sequences or problematic secondary structures, primer extension reactions at elevated temperatures and cloning of blunt-ended amplification products.
- Reduces set-up time and buffer-dye mixing
- No additional loading dye needed – direct loading of final products onto gels
Quality Control
All preparations are assayed for contaminating endonuclease, exonuclease, and non-specific DNase activities. Functionally tested in DNA amplification.
Storage & Stability
- Stable at -20°C for 18 months or at 4°C for 6 months if properly stored.
- Stable for 20 freeze-thaw cycles. To avoid frequent freeze-thaw, keeping small aliquots at -20°C is recommended.
- For daily use, keeping aliquots at 4°C is recommended.
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Amplification of 1.5kb DNA fragment from pTZ DNA region using 2X ViRed Pfu Master Mix in a 50µl reaction mixture (1.0% TBE agarose gel). |
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Lane M : VC 1kb DNA Ladder Lane 1 : DNA amplification product generated with 1u of Pfu DNA Polymerase Lane 2 : DNA amplification product generated with 2X ViRed Pfu Master Mix (store at -20°C) Lane 3 : DNA amplification product generated with 2X ViRed Pfu Master Mix (after 20 freeze-thaw cycles) |
Lane M : VC 1kb DNA Ladder Lane 4 : DNA amplification product generated with 1u of Pfu DNA Polymerase Lane 5 : DNA amplification product generated with 2X Pfu Master Mix (store at -20°C) Lane 6 : DNA amplification product generated with 2X Pfu Master Mix (after 20 freeze-thaw cycles) |
Ordering Information
| Catalog No | Description | Pack Size |
| CLMM03 | 2X ViRed Pfu Master Mix | 100 reactions |
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