Description
2X Pfu Master Mix is an optimized ready-to-use 2X concentrated DNA amplification mixture containing Pfu DNA Polymerase, reaction buffer, dNTPs and enhancers. It contains all the components required for routine DNA amplification except template and primers. Pfu DNA Polymerase is an extremely thermostable proofreading DNA polymerase. It exhibits the 3’ to 5’ proofreading activity, resulting in over 10-fold higher fidelity than possible with Taq DNA Polymerases
Features
- Saves time and reduces contamination due to reduced number of pipetting steps.
- Recommended for use in high-fidelity amplification, amplification of GC-rich sequences or problematic secondary structures, primer extension reactions at elevated temperatures and cloning of blunt-ended amplification products.
- Stable at 4˚C for 6 months, allowing immediate reaction setup without the time-consuming thawing of reagent.
- Suitable for all routine DNA amplification applications.
Quality Control
All preparations are assayed for contaminating endonuclease, exonuclease, and non-specific DNase activities. Functionally tested in DNA amplification.
Storage & Stability
- Stable at -20°C for 18 months or at 4°C for 6 months if properly stored.
- Stable for 20 freeze-thaw cycles. To avoid frequent freeze-thaw, keeping small aliquots at -20°C is recommended.
- For daily use, keeping aliquots at 4°C is recommended.
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Amplification of 1.5kb DNA fragment from pTZ DNA using 2X Pfu Master Mix in a 50μl reaction mixture. |
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1% TBE agarose gel. |
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Lane M : VC 1kb DNA Ladder. Lane 1 : DNA amplification product generated with 1u of Pfu DNA polymerase. Lane 2 : DNA amplification product generated with 2X Pfu Master Mix (store at -20°C). Lane 3 : DNA amplification product generated with 2X Pfu Master Mix (after 20 freeze-thaw cycles).
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Ordering Information
| Catalog No | Description | Pack Size |
| PLMM03 | 2X Pfu Master Mix | 100 reactions |
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