2X ViRed Taq Master Mix

Description
2X ViRed Taq Master Mix is an optimized ready-to-use 2X concentrated DNA amplification mixture premixed with single color dye – red color tracking dye. The 2X ViRed Taq Master Mix contains Taq DNA Polymerase, reaction buffer, dNTPs, MgCl₂, inert red dye and stabilizers needed for routine DNA amplification to obtain a wide range of PCR and DNA products up to 8kb. An inert red dye and stabilizers allows direct loading of final products on t o gels for electrophoresis .This red color dye will not affect the amplification as well as other downstream applications. The red color dye migrates at approximately 400bp on 1% agarose in 1X TBE Buffer.

Features

Suitable for all routine DNA amplification applications
Stable at 4°C for 6 months, allowing immediate reaction setup without the time-consuming thawing of reagents
Reduces set-up time and buffer-dye mixing
Minimizes potential contamination by eliminating several pipetting steps
Easy confirmation of complete mixing
No additional loading dye needed – direct loading of final products onto gels
Generates mostly 3’dA overhang PCR products which are suitable for TA cloning

Composition
Taq DNA Polymerase , 0.4mM dNTPs and 3.0mM MgCl₂ inert red dye and stabilizers.

Supplied With

50mM MgCl₂
Nuclease-free Water

Quality Control
All preparations are assayed for contaminating endonuclease, exonuclease, and non-specific DNase activities. Functionally tested in DNA amplification.

Storage & Stability

Stable at -20°C for 18 months or at 4°C for 6 months if properly stored.
Stable for 20 freeze-thaw cycles. To avoid frequent freeze-thaw, keeping small aliquots at -20°C is recommended.
For daily use, keeping aliquots at 4°C is recommended.