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Home Dyes Nucleic Acid Dyes ViSafe Red Gel Stain (10000X in water)

ViSafe Red Gel Stain (10000X in water)

Description
ViSafe Red Gel Stain is a stable, sensitive and environmentally safe fluorescent nucleic acid dye for staining double stranded DNA (dsDNA), single stranded DNA (ssDNA) or RNA in agarose gels or polyacrylamide gels.

ViSafe Red Gel Stain is designed to replace the highly toxic ethidium bromide (EtBr). Both red gel stain and EtBr have the same spectra, so the red gel stain able to replace the EtBr without changing existing imaging system. The dye is confirmed by Ames test results that it is impenetrable to latex gloves and cell membranes. By using the suggested working concentrations in gel staining, the red gel stain is proven unable to cross cell membranes; and it is noncytotoxic and nonmutagenic at working concentrations.

ViSafe Red Gel Stain, 10000X in H2O, can be diluted 10000X for precast gel protocol or 3000-3300X for post gel staining. One vial (0.5ml) of 10000X solution can be used for at least 100 minigels either using precast method or poststaining method.

Features
Safer
The red gel stain is non cytotoxic & non mutagenic shown by Ames tests.
Higher sensitivity
More sensitive compared to EtBr or Viva SybrGreen Nucleic Acid Stain.
Extremely stable
Stable at room temperature for long-term storage.Stable to be microwaved or being heated. The working solution is stable at room temperature when kept in dark.
Wide application
Suitable to stain dsDNA, ssDNA and RNA. Suitable to use in agarose gel or polyacrylamide gel. Compatible with downstream applications, such as gel recovery & cloning.
Easy staining protocols
Easy precast gel staining & post-staining procedures.
Compatible with most imaging system
Gel can be viewed with standard UV transilluminator, visible light gel reader, or other gel imaging system.

Storage Buffer
Shipped at ambient temperature Stored at 2-8℃ or -20℃

Recommended Equipment Required But Not Supplied:

  1. ElitePro Single Channel Adjustable Volume Pipette, 1-10uL
    (Hercuvan Cat No: TT-EP-10U)
  2. MiniGEL Electrophoresis System
    (Hercuvan Cat No: TT-HES-1-110V/TT-HES-1-220V)
  3. SafeViewer Blue Light Transilluminator
    (Hercuvan Cat No: TT-BLT-470)

Ordering Information

Catalog No Description Pack Size
SD0103 ViSafe Red Gel Stain (10000X in water) 500μl/pack

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ViSafe Red Gel Stain (10000X in water)

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MSDS

ViSafe Red Gel Stain (10000X in water)

Publication
This Product Has Been Used In:

Vaisusuk K et al. (2022) High prevalence of Plasmodium infection in fighting cocks in Thailand determined with a molecular method. Sciendo JVet Res 66, 373-379, 2022

A.Hanifehnezhad et al. (2020) Characterization of local SARS-CoV-2 isolates and pathogenicity in IFNAR -/- mice, Heliyon, 6 (2020) e05116.

Wongpratate et al. (2020) Genetic Polymorphisms of the Human Cytochrome P450 1A1 (CYP1A1) and Cervical Cancer Susceptibility among Northeast Thai Women, Asian Pacific Journal of Cancer Prevention, 21(1):243-248.

Qattan & Khattab (2019) Molecular Characterization of Streptomyces albogriseolus Excellent Mutants for Neomycin Production, . Journal of Pure and Applied Microbiology, 13(3):1489-1498.

 

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Revongen Corporation Center,

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No. 16, Persiaran Setia Dagang,

Setia Alam, Seksyen U13,

40170 Shah Alam,

Selangor Darul Ehsan,

Malaysia.


Tel: +603-3359 1166

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