Concentration
20-50u/μl
5'…GACNN↓NNGTC…3'
3'…CTGNN↑NNCAG…5'
Reaction Conditions
1X Buffer V5
30mM Tris-acetate (pH 7.9 at 30°C), 10mM Mg-acetate, 60mM K-acetate, and 100μg/ml BSA. Incubate at 65°C.
Storage Buffer
10mM Tris-HCl (pH 7.5), 50mM KCl, 0.1mM EDTA, 7mM 2-mercaptoethanol, 200μg/ml BSA and 50% glycerol. Store at -20°C.
Thermal Inactivation
None.
Ligation / Recutting Assay
After 5-fold overdigestion with BstPA I, less than 5% of the DNA fragments can be ligated.
Overdigestion Assay
An unaltered banding pattern was observed after 1μg of DNA was digested with 20u of BstPAI for 16 hours at 25°C.
Supplied with 10X Buffer V5, 10X Buffer UB and Viva Buffer A. (Diluent)
*Prolong Incubation will result in Star Activity.
![](/images/stories/prod_image/restriction_endonucleases/bstpai pshai.jpg)
Ordering Information
Catalog No | Description | Pack Size |
RE1234 | BstPAI {PshAI} | 500u |
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Manual
BstPAI {PshAI}
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MSDS
BstPAI {PshAI}