Concentration
20-100u/μl
5'…G↓AATTC…3'
3'…CTTAA↑G…5'
Reaction Conditions
1X Buffer EcoRI
50mM Tris-HCl (pH 7.5 at 30°C), 10mM MgCl2, 100mM NaCl, 0.02% Triton X-100, and 100μg/ml BSA. Incubate at 37°C.
Storage Buffer
10mM Tris-HCl (pH 7.6), 50mM NaCl, 0.1mM EDTA, 200μg/ml BSA, 1mM DTT and 50% glycerol. Store at -20°C.
Thermal Inactivation
65°C for 20 minutes.
Ligation / Recutting Assay
After 50-fold overdigestion with EcoR I, 95% of the DNA fragments can be ligated and recut.
Overdigestion Assay
An unaltered banding pattern was observed after 1μg of DNA was digested with 50u of EcoRI for 16 hours at 37°C
Supplied with 10X Buffer EcoRI, 10X Buffer UB and Viva Buffer A. (Diluent)
* High enzyme concentration may result in Star Activity.
Ordering Information
| Catalog No | Description | Pack Size |
| RE1260 | EcoRI | 2500u |
Downloads
Manual
EcoRI
Publication
This Product Has Been Used In:
Babak Elyasi Far et al. (2020) Cloning and expression of Staphylococcus simulans lysostaphin enzyme gene in Bacillus subtilis WB600, Research Square.
