Concentration
10-20u/μl
5'…CCC↓GGG…3'
3'…GGG↑CCC…5'
Reaction Conditions
1X Buffer V5
30mM Tris-acetate (pH 7.9 at 30°C), 10mM Mg-acetate, 60mM K-acetate, and 100μg/ml BSA. Incubate at 25°C.
Storage Buffer
10mM Tris-HCl (pH 7.5), 200mM NaCl, 0.1mM EDTA, 7mM 2-mercaptoethanol, 200μg/ml BSA and 50% glycerol. Store at -20°C.
Thermal Inactivation
65°C for 20 minutes.
Ligation / Recutting Assay
After 5-fold overdigestion with SmaI, more than 90% of the DNA fragments can be ligated and recut.
Overdigestion Assay
An unaltered banding pattern was observed after 1μg of DNA was digested with 20u of SmaI for 16 hours at 25°C.
Supplied with 10X Buffer V5, 10X Buffer UB and Viva Buffer A. (Diluent)
*Blocked by CpG methylation.
Ordering Information
| Catalog No | Description | Pack Size |
| RE1336 | SmaI | 1000u |
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Sma I
