Vivantis Technologies Sdn Bhd

Concentration
10-30u/μl

5'…AT↓TAAT…3'
3'…TAAT↑TA…5'

Reaction Conditions
1X Buffer V4
10mM Tris-HCl (pH 8.5 at 30°C), 10mM MgCl₂, 100mM KCl, and 100μg/ml BSA. Incubate at 37°C.

Storage Buffer
10mM KH₂PO₄ (pH 7.2), 50mM NaCl, 0.1mM EDTA, 7mM 2-mercaptoethanol, 200μg/ml BSA and 50% glycerol. Store at -20°C.

Thermal Inactivation
65°C for 20 minutes.

Ligation / Recutting Assay
After 10-fold overdigestion with VspI, 70% of the DNA fragments can be ligated and recut.

Overdigestion Assay
An unaltered banding pattern was observed after 1μg of DNA was digested with 10u of VspI for 16 hours at 37°C.

Supplied with 10X Buffer V4, 10X Buffer UB and Viva Buffer A. (Diluent)
*Blocked by methylation ATTA^mAT

Ordering Information

Download
Manual
VspI

Download
MSDS
VspI