Vivantis Technologies Sdn Bhd

Concentration
1-10u/μl

5'…GCAATGNN↓…3'
3'…CGTTAC↑NN…5

Reaction Conditions
1X Buffer V2
10mM Tris-HCl (pH 7.5 at 30°C), 10mM MgCl₂, 50mM NaCl, and 100μg/ml BSA. Incubate at 60°C.

Storage Buffer
10mM Tris-HCl (pH 7.5), 200mM NaCl, 0.1mM EDTA, 7mM 2-mercaptoethanol, and 50% glycerol. Store at -20°C.

Thermal Inactivation
None.

Ligation / Recutting Assay
After 10-fold overdigestion with Bse3D I, more than 90% of the DNA fragments can be ligated and recut.

Overdigestion Assay
An unaltered banding pattern was observed after 1μg of DNA was digested with 20u of Bse3D I 16 hours at 60°C.

Supplied with 10X Buffer V2, 10X Buffer UB and Viva Buffer A. (Diluent)

Ordering Information

Download
Manual
Bse3D I {BsrDI}

Download
MSDS
Bse3D I {BsrDI}