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GF-1 AmbiClean Kit (Gel & PCR)

Description
The GF-1 AmbiClean Kit (Gel & PCR) is designed for rapid DNA recovery from agarose gel and PCR clean-up of DNA bands ranging from 100bp to 20kb. Special buffer provide the correct salt concentration and pH for efficient recovery (80-90%) of DNA from both PCR product and agarose gel from TAE or TBE buffers. The kit is well suited for the removal of agarose, excess dNTPs, short oligo fragments, mineral oil, enzymes from a PCR reaction product, proteins after restriction enzyme treatment and dephosphorylation, residual dye and ethidium bromide. This kit also allows for concentration of DNA, changing of buffers and desalting.

Features

  • 90% of recovery achievable
  • Purification process less than 15 minutes
  • High pure DNA ready-to-use for routine molecular biology applications such as restriction enzyme digestion, PCR, ligation, DNA sequencing, probe preparations, etc.

Kit Components

  • Buffer DB
  • Wash Buffer (concentrate)
  • Elution Buffer

Ordering Information

Catalog No Description Pack Size
GF-GC-050 GF-1 AmbiClean Kit (Gel & PCR) 50 preps
GF-GC-100 GF-1 AmbiClean Kit (Gel & PCR) 100 preps
GF-GC-200 GF-1 AmbiClean Kit (Gel & PCR) 200 preps

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GF-1 AmbiClean Kit (Gel & PCR)

Publication
This Product Has Been Used In:

Chong, S.Y., Rao, P.V. and Soon, Jan Mei (2017) Chong, S.Y., Rao, P.V. and Soon, Jan Mei International Food Research Journal, 24 (4). pp. 18111818

Garbaj, A.M., Abolghait, S.K., Lawila, A.F., Azwai, S.M., Naas, H.T., Moawad, A.A., Gammoudi, F.T., Barbieri, I., Abureema, S., Eldaghayes, I. (2017). Molecular Identification, Prevalence and Antimicrobial Susceptibility Profile of Cronobacter spp. Cultivated on a Chromogenic Medium in Libya,Journal of Molecular Microbiology, Vol. 1, No. 1 (2017).

Pasandideh, R., Nassiri, B., Shapouri, M.R.S.A., Fayazi, J., Roshanfeker, H., Lotfi, M (2017). Expression of the G1 Epitope of Bovine Ephemeral Fever Virus G Glycoprotein in Eukaryotic cells, Bulgarian Journal of Veterinary Medicine, 1311-1477 (2017).

Said, M.B., Belkahia, H., Mabrouk,N.E., Saidani, M., Hassen, M.B., Alberti, A., Zobba, R., Bouattour, S., Bouattour, A., Messadi, L. (2017) Molecular typing and diagnosis of Anaplasma spp. closely related to Anaplasma phagocytophilum in ruminants from Tunisia. Ticks and Tick-borne Diseases.8. Pp..412-422

Said, M.B., Belhakia, H., Mabrouk, N.E., Saidani, M., Alberti ,A., Zobba,R., Cherif, A., Mahjoub, T., Bouattour, A., Messadi, L. (2017) Anaplasma platys-like strains in ruminants from Tunisia. Infection, Genetics and Evolution 49. Pp..226-233.

Shamsi, H., Mardani, K., Ownagh, A. (2017). Phylogenetic analysis of Escherichia coli isolated from broilers with colibacillosis based on gyrA gene sequences. The Canadian Journal of Veterinary Research, Vol (81), 28-32 (2017).

Azwai, S.M. et al. (2016) Isolation and Molecular Identification of Vibrio spp. By sequencing of 16S rDNA from seafood, meat and meat products in Libya Open Veterinary Journal, 6(1), p. 36-43.

Sriwichai, M., Malem, F., Pholchan, M.K., Bovonsombut, S. (2017). Detection of Bacterial Communities in Volatile-organic-compound (VOC)-contaminated Soil in an Industrial Estate in Eastern Thailand by PCR-DGGE Analysis, Chiang Mai Journal of Science, Vol. 44, No. 3, 742-750 (2017).

Abadi, Z.S.N., Fatemi, F., Khosravani, M., Rahmati, M., Sadroddiny, E (2015). Molecular Cloning of Bone Morphogenetic Protein-2 (BMP- 2) in pGEM-b1 Vector and Transformation into E.coli Rosetta Strain, International Journal of Health Sciences, Vol. 1, No. 3 (2015).

Belkahia, H., Said, M.B., Alberti, A., Abdi, K., Issaoui, Z., Hattab, D., Ghabi, M., Messadi, L. (2015) First molecular survey and novel genetic variants’ identification of Anaplasma marginale, A. centrale and A. bovis in cattle from Tunisia. Infection, Genetics and Evolution. 34. Pp.361-371

Belkahia, H., Said, M.B., Sayahi, L., Alberti, A., Messadi, L. (2015). Detection of novel strains genetically related to Anaplasma platys in Tunisian one-humped camels (Camelus dromedarius), The Journal of Infection in Developing Countries, Vol. 9, No. 10, 1116-1125

Barghi, N., Concepcion, G. P., Olivera, B. M., Lluisma, A.O. (2015). Comparison of the Venom Peptides and Their Expression in Closely Related Conus Species: Insights into Adaptive Post-speciation Evolution of Conus Exogenomes, Genome Biology and Evolution, Vol. 7, No. 6, 1797-1814 (2015).

Deesuth, O., Laopaiboon, P., Klanrit, P., Laopaiboon, L. (2015) Improvement of ethanol production from sweet sorghum juice under high gravity and very high gravity conditions: Effects of nutrient supplementation and aeration. Industrial Crops and Products. 74. Pp.95-102.

Emine, S., Zainol, N., Salihon, J., Convey, P (2015). Mangrove Rhizosphere Soils: A Unique Natural Source of Pravastatin-Producing Penicillium Microfungi, International Journal of Extensive Research, No. 5, 79-87 (2015).

Hamidinejat, H., et al. (2015) Development of an Indirect ELIS using Different Fragments of Recombinant Ncgra 7 for Detection of Neospora caninum Infection in Cattle and Water Buffalo. OIran Journal of Parasitology. 10(1), p.69-77.

Khalafalla, A.I., Al-Busada, K.A., & El-Sabagh, I.M. (2015) Multiplex PCR for Rapid Diagnosis and Differentiation of Pox and Pox-like Diseases in Dromedary Camels. Virology Journal. 12(102), p. 1-10.

Nasanit, R., et al (2015) Assesment of Epiphytic Yeast Diversity in Rice (Oryza sativa) Phyllospehere in Thailand by a Culture-independent Approach Antonie van Leeuwenhoek.Springer. 107(6), p.1475-1490

Said, M.B., Belkahia, H., Karaoud, M., Bousrih, M., Yahiaoui, M. Daaloul-Jedidi, M., Messadi, L. (2015) First molecular survey of Anaplasma bovis in small ruminants from Tunisia. Veterinary Microbiology. 179. Pp.322-326.

Seydametov, E (2015). Novel Pravastatin-Producing Penicillium janthinellum Strain Isolated from Soil, International Journal of Bioscience, Biochemistry and Bioinformatics, Vol. 6, No. 2 (2015).

Wiparat,S., Poeaim, S., Eiamampai, K., Atittayawan (2015). Gender Identification of Himantopus Himantopus Using PCR-Based Method, International Journal of Agricultural Technology, Chiang Mai Journal of Science, Vol. 11, No.2, 307-314 (2015).

Belkahia, H., Said, M.B., Hamdi, S.E., Yahiaoui, M., Gharbi, M., Daaloul-Jedidi, M., Mhadbi, M., Jedidi, M., Darghout, M.A., Klabi, I., Zribi, L., Messadi, L. (2014) ) First molecular identification and genetic characterization ofAnaplasma ovis in sheep from Tunisia. Small Ruminant Research. 121. Pp.404-410.

Mahboubi, M., Falsafi, T., Sadeghizadeh, M (2014). Cloning and Sequence Analysis of Gene Encoding OipA from Iranian Clinical Helicobacter pylori, Iranian Journal of Biotechnology, Vol 12, No. 4, 10-16 (2014).

Şakalar, Ç., Kuk, S., Erensoy, A., Dağli, A.F., Özercan, İ., Çetikaya, Ü, Yazar, S. (2014). Molecular discrimination of Echinococcus granulosus and Echinococcus multilocularis by sequencing and a new PCR- RFLP method with the potential use for other Echinococcus species, Turkish Journal of Medical Sciences, Vol. 44, 741-748 (2014).

Xu, Z., Zikos, D., Tamošiunaite, A., Klopfleisch, R., Osterrieder, N., Tischer, B.K. (2014). Identification of 10 Coxpox Virus Proteins that are necessary for Induction of Hemorrhagic Lesions (Red Pocks) on Chorioallantoic Membranes,Vol. 88, No. 15 (2014).

Nayabian, H., Mardani, K. (2013). Molecular characterization of the chicken anaemia viruses isolated from broiler farms of west Azerbaijan, Iran, Avian Pathology, Vol. 42, No. 2, 108-113 (2013).

Supathaweewat, K., Klanrit, P (2013). cDNA cloning and expression analyses of phytoene synthase 1, phytoene desaturase and -carotene desaturase genes from Solanum lycopersicum KKU-T34003, Songklankarin Journal of Science and Technology, Vol. 35, No. 5, 517-527 (2013).

 

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