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Home DNA Amplification Products 2X Taq Master Mix

2X Taq Master Mix

2X Taq Master Mix is an optimized ready-to-use 2X concentrated DNA amplification mixture containing Taq DNA polymerase, reaction buffer, dNTPs and MgCl2. It contains all the components required for routine DNA amplification, except template and primers.


  • Saves time and reduces contamination due to reduced number of pipetting steps.
  • Stable at 4°C for 6 months, allowing immediate reaction setup without the time consuming thawing of reagents.
  • Suitable for all routine DNA amplification applications.

Taq DNA Polymerase (0.05u/μl), 2X ViBuffer A (100mM KCl, 20mM TrisHCl (pH9.1 at 20°C) and (0.02% Triton™ X-100), 0.4mM dNTPs and 3.0mM MgCl2.

Supplied With

  • 50mM MgCl2
  • Nuclease-free Water

Quality Control
All preparations are assayed for contaminating endonuclease, exonuclease, and non-specific DNase activities. Functionally tested in DNA amplification.

Storage & Stability

  • Stable at -20°C for one year or at 4°C for 6 months if properly stored.
  • Stable for 20 freeze-thaw cycles. To avoid frequent freeze-thaw, keeping small aliquots at -20°C is recommended.
  • For daily use, keeping aliquots at 4°C is recommended.

Ordering Information

Catalog No Description Pack Size
PLMM01 2X Taq Master Mix 100 applications


2X Taq Master Mix

Comparison Test Report

2X Taq Master Mix

This Product Has Been Used In:

Abdul Manas NH et al. (2020) Effects of oil substrate supplementation on production of prodigiosin by Serratia nematodiphila for dye-sensitized solar cell, Journal of Biotechnology, 317:16-26.

Bacus, MG et al. (2020) Global genetic patterns reveal host tropism versus cross-taxon 1 transmission of bat Betacoronaviruses, bioRxiv,

Erkihun A & Kausalya R (2020) MCR-1 Gene Encoded Colistin-Resistant Escherichia coli in Raw Chicken Meat and Bean Sprouts in Malaysia, International Journal of Microbiology, Article ID 8853582.

Elv Nhiel Salo & Annabelle Novero (2020)Identification and Characterisation of Endophytic Bacteria from Coconut (Cocos nucifera) Tissue Culture, Tropical Life Sciences Research,31(1), 2020.

Fatin Aina Zulkhairi Amin et al (2019) Probiotic Properties of Bacillus Strains Isolated from Stingless Bee (Heterotrigona itama) Honey Collected across Malaysia, . International Journal of Environmental Research and Public Health, 17: 278.

Manigandan, G. et al. (2019) Isolation of Pesticide Degrading Bacteria From Paddy Fields and Evaluation of Its Bioremediation Potential Efficiency, . Malaysian Journal of Soil Science, 23:173-182.

Mostafa M. Helal (2019) Association Between Growth Hormone Receptor Gene Polymorphism and Body Weight in Growing Rabbits, . Advances in Animal and Veterinary Sciences,7(11):994-998.

Sandoval, R.F.C. & Cumagun, C.J.R. (2019) Phenotypic and Molecular Analyses of Rhizoctonia spp. Associated with Rice and Other Hosts, . Microorganisms, 7:88.

Boonyayatra, S., Tharavichitkut, P., Oliver, S.P. (2018). Virulence-associated genes and molecular typing of Streptococcus uberis associated with bovine mastitis in northern Thailand, Turkish Journal of Veterinary and Animal Sciences, Vol. 42, 73-81 (2018)

Azhar, N.S., Zin, N.H.M., Abdul-Hamid, T.H.T. (2017). Lactococcus Lactis Strain A5 Producing Nisin-like Bacteriocin Active against Gram Positive and Negative Bacteria, Tropical Life Sciences Research, Vol. 28, No. 2 (2017).

Ajijolakewu, K.A., Leh, C.P., Wan-Abdullah, W.N., Lee, C.K. (2016). Assessment of the Effect of Easily-metabolised Carbon Supplements on Xylanase Production by Newly Isolated Trichoderma asperellum USM SD4 Cultivated on Oil Palm Empty Fruit Bunches. BioResources,Vol. 11, No. 4, 9611-9627 (2016).

Mohamad, Y., Reda, W.W., Abdel-Moein, K., El-Razil, K.A.A., Barakat, A.M.A., El Fadaly, H.A., Hassanain, N.A., Hegazi, A.G. (2016) Prevalence and phylogenetic characterization of Listeria Monocytogenes isolated from processed meat marketed in Egypt.. Journal of Genetic Engineering and Biotechnology. 14(1). Pp.119-123

Allahverdi, A., et al. (2015) Differentiation of Human Mesenchymal Stem Cells into Insulin Producing Cells by Using A Lentiviral Vector Carrying PDX1. CELL Journal.17(2), p.231-242.

Feh, C.F., Wang, K.C., Lu, C.Y., Chiang, L.C., Shieh, D.E., Yen, M.H., Chang, J.S. (2015) Yakammaoto inhibits enterovirus 71 infection by reducing viral attachment, internalization, replication, and translation. Kaohsiung Journal of Medical Sciences. 31. Pp.293-302.

Sabourmoghaddam, N. Zakaria, M.P., Omar, D. (2015) Evidence for the microbial degradation of imidacloprid in soils of Cameron Highlands. Journal of Saudi Society of Agricultural Sciences.14(2) pp.182-188.

Sabourmoghaddam, N. et al.(2015) Evidence for Microbial Degradation of Imidacloprid in Soils of Cameron Highlands. Journal of Saudi Society of Agricultural Sciences. ScienceDirect. 14(2), p. 182-188.

Wiparat,S., Poeaim, S., Eiamampai, K., Atittayawan (2015). Gender Identification of Himantopus Himantopus Using PCR-Based Method, International Journal of Agricultural Technology, Chiang Mai Journal of Science, Vol. 11, No.2, 307-314 (2015).

Belgini, D.R.B., et al (2014) Culturable Bacterial Diversity from a Feed Water of a Reverse Osmosis System, Evaluation of Biofilm Formation and Biocontrol Using Phages. World Journal of Microbiology and Biotechnology. ProQuest. 30, p. 2689-2700.

Belkahia, H., Said, M.B., Hamdi, S.E., Yahiaoui, M., Gharbi, M., Daaloul-Jedidi, M., Mhadbi, M., Jedidi, M., Darghout, M.A., Klabi, I., Zribi, L., Messadi, L. (2014) ) First molecular identification and genetic characterization ofAnaplasma ovis in sheep from Tunisia. Small Ruminant Research. 121. Pp.404-410.

Dhiritiman, C., Sharma, G.D., Jha, D.K., Hijri, M. (2014). Associations of Arbuscular Mycorrhizal (AM) fungi in the Phytoremediation of Trace Metal (TM) Contaminated Soils, Journal of Research in Biology, Vol. 4, No. 2 (2014).

Reyes, J.C.B., Solon, J.A.A., Rivera, W.L. (2014) The analysis of correlation between IL-1B gene expression and genotyping in multiple sclerosis patients. Microbiology and Infectious Disease 179. Pp..337-341.

Hipol, R.M. (2012) Molecular Identification and Phylogenetic Affinity of Two Growth Promoting Fungal Endophytes of Sweet Potato (Ipomea batatas (L.) Lam.) from Baguio City, Philippines. Electric Journal of Biology, 8(3): 57-61.






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