Looking for reliable, safe, nonmutagenic qPCR Dyes for your real-time PCR protocol?
Discover how Viva qGreen Fluorescent Dyes from Vivantis can elevate your qPCR experiments with precision and reliability.
Viva qGreen I Fluorescent Dye (20x in DMSO)
Viva qGreen I Fluorescent Dye (equivalent to SYBR® Green Dye) is a sensitive green fluorescent nucleic acid dye used for detection of double stranded DNA. The dye is widely used in non-specific detection of amplification in quantitative real-time PCR (qPCR) experiments. The detection is monitored by measuring the increase in fluorescence throughout the cycles.
Features:
- Easy and affordable: Probes are not required, reduce assay setup and running cost; given that PCR primers are well designed and reaction is well characterized.
- High sensitivity: Increased fluorescence when bound to any double-stranded DNA.
- Highly stable: Stable during storage and under PCR condition, able to withstand repeated freeze-thaw cycles.
- Versatile applications: Can be used as a general double stranded DNA binding dye for common DNA quantification, melt curve analysis, etc.
- Compatible with most system: Compatible with major brands of qPCR instruments & enzyme systems.
Viva qGreen II Fluorescent Dye (20x in Water)
Viva qGreen II Fluorescent Dye (equivalent to EvaGreen® Dye) is one of the most sensitive dyes to detect double stranded DNA in quantitative real-time PCR (qPCR) experiments as well as high-resolution DNA melt curve analysis, yielding robust and reproducible results.
Features:
- Safer: The dye is noncytotoxic & nonmutagenic for safe handling and easy disposal down to drain, completely impermeable to cell membrane.
- Higher sensitivity: Low PCR inhibitory and high concentration of dye used for maximal signal and high resolution DNA melt analysis.
- Extremely stable: Stable during storage and under PCR condition. No dye decomposition in PCR buffer at 95-100°C for 48 hours. Highly stable under alkaline or acidic condition and able to withstand repeated freeze-thaw cycles.
- Versatile applications: Used as a general double stranded DNA binding dye for DNA quantification, melt curve analysis and more.
- Excellent for qPCR and isothermal application: Brighter and more sensitive than Viva qGreen I Fluorescent Dye (equivalent to SYBR® Green) for detecting amplification due to novel ‘release on demand’ DNA binding mechanism
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