Vivantis Technologies Sdn Bhd

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Home DNA Amplification Products Viva cDNA Synthesis Kit

Viva cDNA Synthesis Kit

Description
Viva cDNA Synthesis Kit is specially designed to provide reliable synthesis of full-length cDNA. M-MuLV RNase H- synthesizes complementary DNA strand initiating from a specific primer, oligo d(T) or random hexamer. The absence of RNase H enhances the synthesis of long cDNA as the RNA strand does not degrade in DNA-RNA hybrid during first strand cDNA synthesis. This cDNA synthesis kit is readily compatible with various cDNA-dependent downstream applications.

Features

  • Absence of RNase H activity allows high yield of full length cDNA synthesis with RNA templates up to 10kb
  • Wide selection of primers, oligo d(T) or random hexamer
  • Highly compatible with various downstream applications
  • Allows synthesis of full length cDNA from various RNA templates
  • High capacity and able to copy up to 2μg of purified mRNA

Applications

  • Traditional PCR amplification or real-time PCR quantification
  • Storage of selective and functional genetic information by removing of introns
  • Cloning of the gene of interested enables horizontal gene transfer (HGT)
  • cDNA probe library construction with large and full-length inserts
  • cDNA microarray for gene discovery and clinical diagnosis via gene pattern screening
  • mRNA expression studies and storage of proteins material at genomics level

Quality Control
All preparation is assayed for contaminating endonucleases, exonucleases and non-specific RNase activities.

Kit Components
Kit Components 50 reactions 100 reactions
M-MuLV Reverse Transcriptase 5,000u 10,000u
10X Buffer M-MuLV 125μl 0.25ml
10mM dNTPs mix 125μl 0.25ml
Oligo d(T)18 (40μm) 50 μl 100μl
Random hexamer (50ng/μl) 50 μl 100μl
Nuclease-free water 1ml 1ml x 2

Storage & Stability
Stable at -20°C for 18 months if reagents are properly stored.

Ordering Information
Catalog No Description Pack Size
cDSK01-050 Viva cDNA Synthesis Kit 50 reactions
cDSK01-100 Viva cDNA Synthesis Kit 100 reactions

Download
Manual

Viva cDNA Synthesis Kit-50 reaction
Viva cDNA Synthesis Kit-100 reaction

Publication
This Product Has Been Used In:

Pazhouhi, M., Sariri, R., Rabzia, A., Khazaei, M. (2016). Thymoquinone synergistically potentiates temozolomide cytotoxicity through the inhibition of autophagy in U87MG cell line, Iranian Journal of Basic Medical Sciences, Vol. 19, 890-898 (2016).

Alavijeh, M.G., Moslemi, E., Izadi, A. (2014). Study the expression of estrogen receptor alpha in women with breast cancer, Bulletin of Environment, Pharmacology and Life Sciences, Vol. 3, No. 12 (2014).

 

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