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Home Latest news New Launch of Vivantis Fast Digest Restriction Endonucleases

New Launch of Vivantis Fast Digest Restriction Endonucleases

14 Feb 2017

Vivantis is honored to announce the release of Fast Digest Restriction Endonucleases – hosted with 24 enzymes under Restriction Endonuclease product line for rapid DNA digestion within 5 to 15 minutes.

Vivantis Fast Digest Restriction Endonucleases are the advanced version of Vivantis restriction endonucleases - designed for rapid DNA digestion within 5-15 minutes. The fast digest restriction endonucleases are suitable for complete digestion of different DNA types, including lambda DNA, plasmid DNA, genomic DNA as well as downstream PCR products.

Each Fast Digest Restriction Endonuclease is supplied with universal buffer, Buffer FD (Buffer Fast Digest), which does not limit multiple digestions of the Fast Digest Restriction Endonucleases. All Fast Digest Restriction Endonucleases are 100% active in Buffer FD, which allows any combination of Fast Digestion Restriction Enzymes to work simultaneously in one reaction tube.

Fast Digest Restriction Endonucleases are experimentally tested and proven to have the same performance as per conventional restriction endonucleases – with lesser reaction times which greatly improve restriction digestion efficiency.


APPLICATIONS:

  • Digestion of different types of DNAs
  • Digestion of PCR products
  • RFLP genotyping
  • Clone analysis


Hassle-Free Restriction Digestion within 5-15 Minutes

  • NO MORE long incubation time and overnight digestions
  • NO MORE various color tag buffer system
  • NO MORE dilution
  • NO MORE star activity

Summary Table of Incubation Time of Vivantis Fast Digest Restriction Endonucleases

Cat. No. Fast Digest Restriction Endonuclease Incubation time (mins) in 20μl reaction volume Incubation time (mins) in 50μl reaction volume
5 10 15 5 10 15
FDRE1104 Acc65 I
FDRE1118 Ahl I
FDRE1120 Alu I
FDRE1124 Apa I
FDRE1126 AsiG I
FDRV1138 BamH I
FDRV1144 Bgl II
FDRE1226 BstMA I
FDRE1260 EcoR I
FDRE1262 EcoR V
FDRE1266 FauND I
FDRV1276 Hind III
FDRE1278 Hinf I
FDRE1280 Hpa I
FDRE1294 Mlu I
FDRE1308 Pce I
FDRE1312 Psp124B I
FDRE1320 Pst I
FDRV1326 Sal I
FDRE1332 Sfr274 I
FDRE1336 Sma I
FDRV1340 Sph I
FDRV1364 Xba I

Comparison between Vivantis Restriction Endonucleases and Vivantis Fast Digest Restriction Endonucleases

Vivantis Restriction Endonuclease Vivantis Fast Digest Restriction Endonuclease (FDRE)
Incubation time 1 hour – 16 hours 5 – 15 minutes
Buffer system
Up to 15 color-tag reaction buffers
(eg. Buffer V1, V2, UB1x, etc)
One universal buffer (Buffer FD)
  • act as a reaction buffer AND universal buffer, suitable for all types of fast digest RE
  • able to perform double and multiple digestion of any combination of fast digest RE
Double/multiple restriction digestion Limited due to buffer incompatibility No limit – all 24 Fast Digest Restriction Endonucleases are 100% active in one universal buffer
Dilution step Dilution is optional depending on experiment NO dilution required
Activity definition 1 unit of restriction enzyme is required to digest 1ug of DNA in 1 hour at optimum temperature in 50µl of assay buffer. 1µl of restriction enzyme is required to digest 1µg of DNA within 15 minutes at optimum temperature in 20µl or 50µl of assay buffer.
 

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