Description
The ViPrimePLUS Chikungunya Virus RT-qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of Chikungunya virus genomes in clinical samples (e.g. blood, plasma and serum).
Chikungunya virus (CHIKV) is an arbovirus that causes arthralgia, arthritis, fever and other systemic symptoms, resulting in stooped appearance of sufferers with joint pain. Although the infection lasts only two to five days, some cases may persist for weeks or months. Other possible complications that may arise involve the eyes, nervous system and gastrointestinal tract. Severe untreated cases may lead to death. It is often confused with the classic dengue virus infection due to similar clinical symptoms. It is carried and transmitted by Aedes mosquitoes to humans. Thus, real-time PCR test plays a major role in this area.
ViPrimePLUS Chikungunya Virus RT-qPCR Kit is intended for research use only.
Kit Component
- CHIKV Primers and Probe Mix (CHIKV PPM)
- Positive Control
- Internal Extraction Control Primers and Probe Mix (IEC PPM)
- Internal Extraction Control (IEC DNA)
- Nuclease Free Water
- Template Preparation Buffer
Product Specification
| Technology | Taqman probe-based real-time PCR assay |
| Type of Nucleic Acid Kit | RNA |
| Kit Storage | Shipped in room temperature, store at -20℃ on arrival |
| Sample Material | Clinical samples (e.g. blood, plasma, serum) |
| Gene Target | Non structural protein 2 (nsp2) |
| Detection Limit | 10 to <100 copies per reaction |
| Sensitivity & Specificity | Ct value 28±3 with probability of 95% |
| Controls Included | • Internal Extraction Control (IEC) as inhibition control • Positive control |
| Channels | • FAM channels detect pathogen amplicons • VIC/HEX channel detects IEC amplicons |
Ordering Information
| Catalog No | Description | Pack Size |
| QM5004 | ViPrimePLUS Chikungunya Virus RT-qPCR Kit | 150 reactions |
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Manual
