Description
The ViPrimePLUS H1N1 Influenza Human Pandemic Strain RT-qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of H1N1 Influenza Human Pandemic Strain virus genome in clinical samples (e.g. nasopharyngeal swab and blood).
Influenza virus is a RNA virus that infects birds and mammals. Transmission is via contact with bodily fluid of infected person or animals. Symptoms of influenza disease are often confused with the less severe common cold, such as fever, sore throat, muscle and headaches, cough and fatigue. In persistent infection, it could develop into pneumonia that is fatal to children and elderly. The 3 types of influenza are influenza A, influenza B and the low epidemiological concern influenza C. Subtypes are termed based on the surface glycoprotein types, i.e. hemagglutinin (H) and neuraminidase (N). In human, type A and B are the common typical seasonal flu, resulting in 300,000 deaths yearly and up to millions in pandemic years. Pandemic usually occurs when the influenza virus acquire ability via mutation and gene reassortment to cross-infect between animals and human. Influenza A consists of only 1 species and infects birds and some mammals. Examples of major influenza A pandemics are the 2009 H1N1 flu (swine flu) and 2003 H5N1 flu (avian influenza) outbreaks, recording more than 50% mortality rate.
ViPrimePLUS H1N1 Influenza Human Pandemic Strain RT-qPCR Kit is intended for research use only.
Kit Component
- M1 (global) , N1 (H1N1) Primers and Probe Mix (M1 (global) , N1 (H1N1) PPM)
- M1 and N1 Positive Control
- Internal Extraction Control Primers and Probe Mix (IEC PPM)
- Internal Extraction Control (IEC DNA)
- Nuclease Free Water
- Template Preparation Buffer
Product Specification
| Technology | Taqman probe-based real-time PCR assay |
| Type of Nucleic Acid Kit | RNA |
| Kit Storage | Shipped in room temperature, store at -20℃ on arrival |
| Sample Material | Clinical samples (e.g. nasopharyngeal swab, blood) |
| Gene Target | M1 gene (human and swine) and N1 gene (human only) |
| Detection Limit | 10 to <100 copies per reaction |
| Sensitivity & Specificity | Ct value 28±3 with probability of 95% |
| Controls Included | • Internal Extraction Control (IEC) as inhibition control • Positive control |
| Channels | • FAM channels detect pathogen amplicons • VIC/HEX channel detects IEC amplicons |
Ordering Information
| Catalog No | Description | Pack Size |
| QM5076 | ViPrimePLUS H1N1 Influenza Human Pandemic Strain RT-qPCR Kit | 150 reactions |
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Manual
ViPrimePLUS H1N1 Influenza Human Pandemic Strain RT-qPCR Kit
