Description
The ViPrimePLUS Hepatitis C Virus RT-qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of Hepatitis C Virus genome in clinical samples (e.g. blood plasma, serum and liver biopsy).
Hepatitis C virus is a small, enveloped, single stranded RNA virus. About 3-4million people worldwide are infected with the virus each year and 350 000 deaths resulted from it. The virus is transmitted through contact with infected blood. Hepatitis C causes both acute and chronic infection which leads to liver cirrhosis, liver cancer and fatal liver failure. Because of its severity, Hepatitis C disease is the main reason for liver transplants in US. Vaccines have yet to be developed due to the highly mutable RNA genome. Anti-viral drug are currently in the stages of clinical studies. Thus, fast and accurate detection is essential in preventing the irreversible liver damage.
ViPrimePLUS Hepatitis C Virus RT-qPCR Kit is intended for research use only.
Kit Component
- HCV Primers and Probe Mix (HCV PPM)
- Positive Control
- Internal Extraction Control Primers and Probe Mix (IEC PPM)
- Internal Extraction Control (IEC DNA)
- Nuclease Free Water
- Template Preparation Buffer
Product Specification
| Technology | Taqman probe-based real-time PCR assay |
| Type of Nucleic Acid Kit | RNA |
| Kit Storage | Shipped in room temperature, store at -20℃ on arrival |
| Sample Material | Clinical samples (e.g. blood plasma, serum, liver biopsy) |
| Gene Target | 5’ untranslated region |
| Detection Limit | 10 to <100 copies per reaction |
| Sensitivity & Specificity | Ct value 28±3 with probability of 95% |
| Controls Included | • Internal Extraction Control (IEC) as inhibition control • Positive control |
| Channels | • FAM channels detect pathogen amplicons • VIC/HEX channel detects IEC amplicons |
Ordering Information
| Catalog No | Description | Pack Size |
| QM5012 | ViPrimePLUS Hepatitis C Virus RT-qPCR Kit | 150 reactions |
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Manual
