Description
The ViPrimePLUS Hepatitis E Virus RT-qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of Hepatitis E Virus genome in faecal matter and food or water sample.
Hepatitis E virus is a single stranded RNA virus with a linear genome. There are about 20 million new cases reported each year globally. Transmission is usually through contaminated drinking water, i.e. fecal-oral route. HEV infection is normally self-limiting without lasting damage. However in rare occasion, fulminant hepatitis may develop leading to acute liver failure, with higher prevalence among pregnant ladies. Re-activation of HEV is found in immunocompromised patients, resulting in chronic infection.
ViPrimePLUS Hepatitis E Virus RT-qPCR Kit is intended for research use only.
Kit Component
- HEV Primers and Probe Mix (HEV PPM)
- Positive Control
- Internal Extraction Control Primers and Probe Mix (IEC PPM)
- Internal Extraction Control (IEC DNA)
- Nuclease Free Water
- Template Preparation Buffer
Product Specification
| Technology | Taqman probe-based real-time PCR assay |
| Type of Nucleic Acid Kit | RNA |
| Kit Storage | Shipped in room temperature, store at -20℃ on arrival |
| Sample Material | • Clinical samples (e.g. faecal) • Food samples • Water samples |
| Gene Target | Open reading frame 2 (ORF2) region |
| Detection Limit | 10 to <100 copies per reaction |
| Sensitivity & Specificity | Ct value 28±3 with probability of 95% |
| Controls Included | • Internal Extraction Control (IEC) as inhibition control • Positive control |
| Channels | • FAM channels detect pathogen amplicons • VIC/HEX channel detects IEC amplicons |
Ordering Information
| Catalog No | Description | Pack Size |
| QM5014 | ViPrimePLUS Hepatitis E Virus RT-qPCR Kit | 150 reactions |
Download
Manual
