Description
The ViPrimePLUS Herpes Simplex Virus 1 and 2 qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of Herpes Simplex Virus 1 and 2 genomes in clinical samples (e.g. oral and genital swabs).
Human Herpes virus (HHV) is classified into 8 subtypes with each of them manifesting different disease implications. Herpes Simplex virus 1 and 2 (HSV1 and HSV2) are two much related HHV with 50% similarity in their genomes. HSV1 commonly causes cold sores, fever blisters and to a lesser extent, genital lesions and neonatal herpes. On the other hand, HSV2 is more likely to cause genital lesions, neonatal herpes and very rare cases of oral infections. Most of the infections remained asymptomatic or exhibit very mild symptoms that are often ignored. When symptoms do show, blisters appear in the oral cavity and genital area. In an outbreak (when the blisters rupture), contact with the open sores is highly contagious and risks the transfer of the infection to other parts of the body, especially the eyes which causes ocular herpes and eventually blindness. Recurrent outbreaks are common with diminishing frequency over time. Infection with HHV cannot be fully cleared, thus only treatments for symptoms are available.
ViPrimePLUS Herpes Simplex Virus 1 and 2 qPCR Kit is intended for research use only.
Kit Component
- HSV1&2 Primers and Probe Mix (HSV1&2 PPM)
- Positive Control
- Internal Extraction Control Primers and Probe Mix (IEC PPM)
- Internal Extraction Control (IEC DNA)
- Nuclease Free Water
- Template Preparation Buffer
Product Specification
| Technology | Taqman probe-based real-time PCR assay |
| Type of Nucleic Acid Kit | DNA |
| Kit Storage | Shipped in room temperature, store at -20℃ on arrival |
| Sample Material | Clinical samples (e.g. oral, genital swabs) |
| Gene Target | UL23 thymidine kinase (TK) and UL36 large tegument protein gene of HSV1 and HSV2 |
| Detection Limit | 10 to <100 copies per reaction |
| Sensitivity & Specificity | Ct value 28±3 with probability of 95% |
| Controls Included | • Internal Extraction Control (IEC) as inhibition control • Positive control |
| Channels | • FAM channels detect pathogen amplicons • VIC/HEX channel detects IEC amplicons |
Ordering Information
| Catalog No | Description | Pack Size |
| QM5018 | ViPrimePLUS Herpes Simplex Virus 1 and 2 qPCR Kit | 150 reactions |
Download
Manual
