Description
The ViPrimePLUS Human Influenza B Virus RT-qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of Human Influenza B Virus genome in clinical samples (e.g. nasopharyngeal swab and blood).
Influenza virus is a RNA virus which infects birds and mammals. Transmission is via contact with bodily fluid of infected person or animals. Symptoms of influenza disease are often confused with the less severe common cold, such as fever, sore throat, muscle and headaches, cough and fatigue. In persistent infection, it could develop into pneumonia that is fatal to children and elderly. The 3 types of influenza are influenza A, influenza B and influenza C (less occurrence, sporadic). In human, type A and B are the common typical seasonal flu, resulting in 300,000 deaths yearly and up to millions in pandemic years. Pandemic usually occur when the influenza virus acquire ability via mutation and gene reassortment to cross-infect between animals and human. Influenza B, which consists of only 1 species, infects human and seals. Due to its limited host range and lower evolution rate (2-3 times slower than influenza A yet still faster than influenza C), influenza B pandemic is less epidemic as compared to type A.
ViPrimePLUS Human Influenza B Virus RT-qPCR Kit is intended for research use only.
Kit Component
- FluB Primers and Probe Mix (FluB PPM)
- Positive Control
- Internal Extraction Control Primers and Probe Mix (IEC PPM)
- Internal Extraction Control (IEC DNA)
- Nuclease Free Water
- Template Preparation Buffer
Product Specification
| Technology | Taqman probe-based real-time PCR assay |
| Type of Nucleic Acid Kit | RNA |
| Kit Storage | Shipped in room temperature, store at -20℃ on arrival |
| Sample Material | Clinical samples (e.g. nasopharyngeal swab, blood) |
| Gene Target | Haemoglutinin gene |
| Detection Limit | 10 to <100 copies per reaction |
| Sensitivity & Specificity | Ct value 28±3 with probability of 95% |
| Controls Included | • Internal Extraction Control (IEC) as inhibition control • Positive control |
| Channels | • FAM channels detect pathogen amplicons • VIC/HEX channel detects IEC amplicons |
Ordering Information
| Catalog No | Description | Pack Size |
| QM5033 | ViPrimePLUS Human Influenza B Virus RT-qPCR Kit | 150 reactions |
Download
Manual
