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Home ViPrimePLUS Real-Time PCR Kits Human Infection qPCR/RT-qPCR Kits Virus ViPrimePLUS Human Metapneumovirus RT-qPCR Kit

ViPrimePLUS Human Metapneumovirus RT-qPCR Kit

Description
The ViPrimePLUS Human Metapneumovirus RT-qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of Human Metapneumovirus genome in clinical samples (e.g. sputum, nasopharyngeal aspirate and throat swab).

Human Metapneumovirus (hMPV) is a single negative-strand RNA virus that causes lower respiratory tract infection in human. It is classified into 2 major groups (i.e. A and B) and 4 subgroups. Following behind respiratory syncytial virus, hMPV is the second most commonly identified cause of pediatric lower respiratory illness. In a study, hMPV infection was found in 10% of the lung transplant recipients with acute respiratory tract infections, thus signifying the higher risk of severe hMPV illness in transplant patients. Immunocompromised patients are also susceptible to the severe disease.

ViPrimePLUS Human Metapneumovirus RT-qPCR Kit is intended for research use only.

Kit Component

  • HMPV Primers and Probe Mix (HMPV PPM)
  • Positive Control
  • Internal Extraction Control Primers and Probe Mix (IEC PPM)
  • Internal Extraction Control (IEC DNA)
  • Nuclease Free Water
  • Template Preparation Buffer

Product Specification

Technology Taqman probe-based real-time PCR assay
Type of Nucleic Acid Kit RNA
Kit Storage Shipped in room temperature, store at -20℃ on arrival
Sample Material Clinical samples (e.g. sputum, nasopharyngeal aspiratel, throat swab)
Gene Target Nucleoprotein gene in the RNA genome
Detection Limit 10 to <100 copies per reaction
Sensitivity & Specificity Ct value 28±3 with probability of 95%
Controls Included • Internal Extraction Control (IEC) as inhibition control
• Positive control
Channels • FAM channels detect pathogen amplicons
• VIC/HEX channel detects IEC amplicons

Ordering Information

Catalog No Description Pack Size
QM5035 ViPrimePLUS Human Metapneumovirus RT-qPCR Kit 150 reactions

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ViPrimePLUS Human Metapneumovirus RT-qPCR Kit

 

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