Description
The ViPrimePLUS Japanese Encephalitis Virus RT-qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of Japanese Encephalitis virus genome in clinical samples (e.g. serum blood).
Japanese Encephalitis (JE) is a neurological infection caused by a mosquito-borne, single-stranded RNA virus, which is related to dengue, yellow fever and West Nile viruses. The primary host vector is Culex tritaeniorhynchus. The infection is endemic with seasonal distribution in parts of China, the south-east of the Russian Federation, and the South and South-East Asia. Hyperendemicity is found in the northern, parts of the central and southern India, and southern Nepal. Approximately, 3 billion people residing in those areas were exposed to the threat of JE.
ViPrimePLUS Japanese Encephalitis Virus RT-qPCR Kit is intended for research use only.
Kit Component
- JEV Primers and Probe Mix (JEV PPM)
- Positive Control
- Internal Extraction Control Primers and Probe Mix (IEC PPM)
- Internal Extraction Control (IEC DNA)
- Nuclease Free Water
- Template Preparation Buffer
Product Specification
| Technology | Taqman probe-based real-time PCR assay |
| Type of Nucleic Acid Kit | RNA |
| Kit Storage | Shipped in room temperature, store at -20℃ on arrival |
| Sample Material | Clinical samples (e.g. serum blood) |
| Gene Target | Non-structural protein 5 (ns5) |
| Detection Limit | 10 to <100 copies per reaction |
| Sensitivity & Specificity | Ct value 28±3 with probability of 95% |
| Controls Included | • Internal Extraction Control (IEC) as inhibition control • Positive control |
| Channels | • FAM channels detect pathogen amplicons • VIC/HEX channel detects IEC amplicons |
Ordering Information
| Catalog No | Description | Pack Size |
| QM5059 | ViPrimePLUS Japanese Encephalitis Virus RT-qPCR Kit | 150 reactions |
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