Description
The ViPrimePLUS Cryptosporidium qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of Cryptosporidium genomes in faecal or water samples.
Cryptosporidium is a protozoan parasite that infects the gastrointestinal tract of animals and humans. C. parvum and C. hominis are the common human pathogenic species. C. canis, C. felis, C. meleagridis, and C. muris may cause disease in humans, too. Infection with the protozoa causes watery diarrhea, abdominal cramps and vomiting, but generally self-limiting in healthy individuals. The symptoms are more severe and prolonged in immunocompromised patients. This water-borne parasite is usually transmitted by the intake of untreated drinking water and fecal contaminated water system. There is evidence suggesting the possible airborne transmission especially in hospital setting, causing nosocomial infection. As the parasite is resistant to chlorine, a good filtration system and proper water boiling procedure are necessary steps to remove the oocysts.
ViPrimePLUS Cryptosporidium qPCR Kit is intended for research use only.
Kit Component
- Cryptosporidium Primers and Probe Mix (Cryptosporidium PPM)
- Positive Control
- Internal Extraction Control Primers and Probe Mix (IEC PPM)
- Internal Extraction Control (IEC DNA)
- Nuclease Free Water
- Template Preparation Buffer
Product Specification
| Technology | Taqman probe-based real-time PCR assay |
| Type of Nucleic Acid Kit | DNA |
| Kit Storage | Shipped in room temperature, store at -20℃ on arrival |
| Sample Material | • Clinical samples (e.g. faecal) • Water samples |
| Gene Target | Oocyst wall protein (COWP) gene |
| Detection Limit | 10 to <100 copies per reaction |
| Sensitivity & Specificity | Ct value 28±3 with probability of 95% |
| Controls Included | • Internal Extraction Control (IEC) as inhibition control • Positive control |
| Channels | • FAM channels detect pathogen amplicons • VIC/HEX channel detects IEC amplicons |
Ordering Information
| Catalog No | Description | Pack Size |
| QM4003 | ViPrimePLUS Cryptosporidium qPCR Kit | 150 reactions |
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