Description
The ViPrimePLUS Bacillus cereus E33 qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of Bacillus cereus E33 genomes in faecal, food and water samples.
Bacillus cereus is a Gram positive, mesophilic bacterium which forms endospores. Being a causative agent for the rapid-onset emetic syndrome and slow-onset diarrheal syndrome of food poisoning, it is one of the clinically important pathogen in food industries. Consumption of undercooked meat and food is the main source of infection. The bacteria are capable of producing different types of endotoxins; i.e. Hemolysin BL (HBL), non-hemolytic enterotoxin (Nhe), cytotoxin K (EntK) and the heat-resistant emetic toxin (ETE), which brings about infection and food poisoning. In immunocompromised patients and newborns, systemic and local infection is common too.
ViPrimePLUS Bacillus cereus E33 qPCR Kit is intended for research use only.
Kit Component
- B. cereus E33 primers and Probe Mix (B. cereus E33 PPM)
- Positive Control
- Internal Extraction Control Primers and Probe Mix (IEC PPM)
- Internal Extraction Control (IEC DNA)
- Nuclease Free Water
- Template Preparation Buffer
Product Specification
| Technology | Taqman probe-based real-time PCR assay |
| Type of Nucleic Acid Kit | DNA |
| Kit Storage | Shipped in room temperature, store at -20℃ on arrival |
| Sample Material | • Faecal samples • Food samples • Water samples |
| Gene Target | Phage-related protein gene |
| Detection Limit | 10 to <100 copies per reaction |
| Sensitivity & Specificity | Ct value 28±3 with probability of 95% |
| Controls Included | • Internal Extraction Control (IEC) as inhibition control • Positive control |
| Channels | • FAM channels detect pathogen amplicons • VIC/HEX channel detects IEC amplicons |
Ordering Information
| Catalog No | Description | Pack Size |
| QM2004 | ViPrimePLUS Bacillus cereus E33 qPCR Kit | 150 reactions |
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Manual
