Description
The ViPrimePLUS Bordetella pertussis qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of Bordetella pertussis genomes in clinical samples (e.g. sputum, nasopharyngeal fluid and throat swab).
Bordetella pertussis is a Gram negative coccobacillus pathogen, which causes pertussis, also known as “whooping cough”. Symptoms of the disease are similar to common cold and it sometimes produces a “whooping” sound when inhaling or vomiting. The highly contagious infection is transmitted via respiratory droplets. Pertussis toxin paralyzes the cilia and causes inflammation of the respiratory tract, which resulted in the mucous accumulation in the tract. It is the leading cause of infant's death in the pre-vaccination era. Even since the introduction of vaccination, the number of incidences had reduced by 80%. Given proper treatment and care, prognosis for full recovery from pertussis is good with minimal complications. However, infants younger than 6 months with pertussis may suffer from more severe complications such as pneumonia, lymphocytosis, seizures and encephalopathy, and increase mortality rate.
ViPrimePLUS Bordetella pertussis qPCR Kit is intended for research use only.
Kit Component
- B. pertussis Primers and Probe Mix (B. pertussis PPM)
- Positive Control
- Internal Extraction Control Primers and Probe Mix (IEC PPM)
- Internal Extraction Control (IEC DNA)
- Nuclease Free Water
- Template Preparation Buffer
Product Specification
| Technology | Taqman probe-based real-time PCR assay |
| Type of Nucleic Acid Kit | DNA |
| Kit Storage | Shipped in room temperature, store at -20℃ on arrival |
| Sample Material | Clinical samples (e.g. sputum, nasopharyngeal fluid and throat swab) |
| Gene Target | Phage-related protein gene |
| Detection Limit | 10 to <100 copies per reaction |
| Sensitivity & Specificity | Ct value 28±3 with probability of 95% |
| Controls Included | • Internal Extraction Control (IEC) as inhibition control • Positive control |
| Channels | • FAM channels detect pathogen amplicons • VIC/HEX channel detects IEC amplicons |
Ordering Information
| Catalog No | Description | Pack Size |
| QM2005 | ViPrimePLUS Bordetella pertussis qPCR Kit | 150 reactions |
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