Description
The ViPrimePLUS Campylobacter coli qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of Campylobacter coli genomes in faecal and food samples.
Campylobacter spp. represents a group of Gram negative, spiral-shaped bacteria that infects the gastrointestinal tract causing campylobacteriosis. Transmission of infection is usually via ingestion of contaminated food and water. This foodborne infection causes symptoms of fever, abdominal cramp, bloody diarrhea and dysentery and is usually self-limiting where it will resolve within a week in most healthy person. However, in immunocompromised individuals, Campylobacter often invades the bloodstream causing life-threatening infection. C. jejuni is the main cause of campylobacteriosis, followed by C. coli, a close relative. Therefore, routine screening and good hygiene practice in food handling is essential.
ViPrimePLUS Campylobacter coli qPCR kit is intended for research use only.
Kit Component
- C. coli Primers and Probe Mix (C. coli PPM)
- Positive Control
- Internal Extraction Control Primers and Probe Mix (IEC PPM)
- Internal Extraction Control (IEC DNA)
- Nuclease Free Water
- Template Preparation Buffer
Product Specification
| Technology | Taqman probe-based real-time PCR assay |
| Type of Nucleic Acid Kit | DNA |
| Kit Storage | Shipped in room temperature, store at -20℃ on arrival |
| Sample Material | • Faecal samples • Food samples |
| Gene Target | CadF gene |
| Detection Limit | 10 to <100 copies per reaction |
| Sensitivity & Specificity | Ct value 28±3 with probability of 95% |
| Controls Included | • Internal Extraction Control (IEC) as inhibition control • Positive control |
| Channels | • FAM channels detect pathogen amplicons • VIC/HEX channel detects IEC amplicons |
Ordering Information
| Catalog No | Description | Pack Size |
| QM2010 | ViPrimePLUS Campylobacter coli qPCR Kit | 150 reactions |
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Manual
