Description
The ViPrimePLUS Mycobacterium tuberculosis qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of the Mycobacterium tuberculosis genomes in clinical samples (e.g. sputum, nasopharyngeal fluid and throat swab).
Mycobacterium tuberculosis is a Gram positive, fastidious, obligate aerobe which causes major lower respiratory tract disease, namely tuberculosis. Tuberculosis (TB) is the second lead killer of infectious disease with 1.4 million reported deaths reported in 2011. About a third of the world's population suffered from latent TB, where there are 10% chances for them to fall ill to the disease. Immunocompromised patients and smokers seem to have increased risk for developing active TB infection. The disease starts with mild symptoms thus making it difficult for early treatment. TB and HIV co-infection is a major concern as the patient is 21 to 34 times more likely to develop active TB disease and almost 25% of deaths in AIDS patients are due to TB. The treatment is usually via a course of antibiotic administration. However the evolution of multidrug resistant TB strains has required the use of stronger drugs for treatment.
ViPrimePLUS Mycobacterium tuberculosis qPCR Kit is intended for research use only.
Kit Component
- M. tuberculosis Primers and Probe Mix (M. tuberculosis PPM)
- Positive Control
- Internal Extraction Control Primers and Probe Mix (IEC PPM)
- Internal Extraction Control (IEC DNA)
- Nuclease Free Water
- Template Preparation Buffer
Product Specification
| Technology | Taqman probe-based real-time PCR assay |
| Type of Nucleic Acid Kit | DNA |
| Kit Storage | Shipped in room temperature, store at -20℃ on arrival |
| Sample Material | Clinical samples (e.g. sputum, nasopharyngeal fluid, throat swab) |
| Gene Target | rep13E12 gene |
| Detection Limit | 10 to <100 copies per reaction |
| Sensitivity & Specificity | Ct value 28±3 with probability of 95% |
| Controls Included | • Internal Extraction Control (IEC) as inhibition control • Positive control |
| Channels | • FAM channels detect pathogen amplicons • VIC/HEX channel detects IEC amplicons |
Ordering Information
| Catalog No | Description | Pack Size |
| QM2042 | ViPrimePLUS Mycobacterium tuberculosis qPCR Kit | 150 reactions |
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