Description
The ViPrimePLUS Mycoplasma pneumoniae qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of the Mycoplasma pneumoniae genomes in clinical samples (e.g. nasopharyngeal fluid, sputum and throat swab).
Mycoplasma pneumoniae is a small bacterium which lacks a cell wall, and thus does not Gram stain. It infects the mucosal membrane of the upper and lower respiratory tract, causing dry cough, sore throat, fever and the more serious tracheobronchitis and atypical pneumonia. The incubation period is long with gradual onset of symptoms. Absence of cough at the initial stage often makes diagnosis difficult. The later persistent, progressive cough stage usually goes on for 3 weeks or more. Aside from Chlamydophila pneumoniae and Legionella pneumophila, M. pneumoniae is one of the atypical pathogens that are responsible for the 20-30% cases of the community-acquired pneumonia, particularly in children and adults (typically 40 years old and younger). Patients with sickle cell anemia and young children are more prone to more severe complications. Due to the indistinctive features of the illness, real time PCR detection assay of the pathogen is well sought after.
ViPrimePLUS Mycoplasma pneumoniae qPCR Kit is intended for research use only.
Kit Component
- M. pneumoniae Primers and Probe Mix (M. pneumoniae PPM)
- Positive Control
- Internal Extraction Control Primers and Probe Mix (IEC PPM)
- Internal Extraction Control (IEC DNA)
- Nuclease Free Water
- Template Preparation Buffer
Product Specification
| Technology | Taqman probe-based real-time PCR assay |
| Type of Nucleic Acid Kit | DNA |
| Kit Storage | Shipped in room temperature, store at -20℃ on arrival |
| Sample Material | Clinical samples (e.g. nasopharyngeal fluid, sputum, throat swab) |
| Gene Target | 16S ribosomal RNA gene |
| Detection Limit | 10 to <100 copies per reaction |
| Sensitivity & Specificity | Ct value 28±3 with probability of 95% |
| Controls Included | • Internal Extraction Control (IEC) as inhibition control • Positive control |
| Channels | • FAM channels detect pathogen amplicons • VIC/HEX channel detects IEC amplicons |
Ordering Information
| Catalog No | Description | Pack Size |
| QM2044 | ViPrimePLUS Mycoplasma pneumoniae qPCR Kit | 150 reactions |
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