Description
The ViPrimePLUS Neisseria meningitis qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of the Neisseria meningitidis genomes in clinical samples (e.g. cerebrospinal fluids, throat swabs and blood).
Neisseria meningitidis is a Gram negative bacterium, which causes severe infection in the meninges of the brain. Fatality rate of this bacterial meningitis reaches 50% if left untreated. There are 12 serovars of N. meningitidis, of which 6 can cause epidemic, i.e. A, B, C, W135, X and Y. Transmission of the bacteria is via respiratory droplet, and the pathogen only infects human, thus without animal reservoir. It is estimated that 10-20% of human population are carriers of the bacteria. The infection may leads to brain damage, hearing loss and impaired learning ability. Introduction of the new meningococcal A vaccine has greatly reduces the incidences of infection in many developed countries.
ViPrimePLUS Neisseria meningitis qPCR Kit is intended for research use only.
Kit Component
- N. meningitidis Primers and Probe Mix (N. meningitidis PPM)
- Positive Control
- Internal Extraction Control Primers and Probe Mix (IEC PPM)
- Internal Extraction Control (IEC DNA)
- Nuclease Free Water
- Template Preparation Buffer
Product Specification
| Technology | Taqman probe-based real-time PCR assay |
| Type of Nucleic Acid Kit | DNA |
| Kit Storage | Shipped in room temperature, store at -20℃ on arrival |
| Sample Material | Clinical samples (e.g. cerebrospinal fluids, throat swabs, blood) |
| Gene Target | Superoxide dismutase gene (sodC) |
| Detection Limit | 10 to <100 copies per reaction |
| Sensitivity & Specificity | Ct value 28±3 with probability of 95% |
| Controls Included | • Internal Extraction Control (IEC) as inhibition control • Positive control |
| Channels | • FAM channels detect pathogen amplicons • VIC/HEX channel detects IEC amplicons |
Ordering Information
| Catalog No | Description | Pack Size |
| QM2046 | ViPrimePLUS Neisseria meningitis qPCR Kit | 150 reactions |
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